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Mutation in the GA3ox gene governs short-internode characteristic in a korean cucumber inbred line

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Abstract

Here, we identified a new natural mutant, si-2, from a Korean cucumber inbred line. The genes responsible for dwarf (DW) and/or short internode (SI) in cucurbits were selected and used to identify the gene(s) controlling the si-2 phenotype. We evaluated the transcript levels of the candidate genes between wild type (WT) and si-2 mutant and identified sequence polymorphism from the differentially expressed genes between the WT and si-2 mutants. Finally, we identified that CsGy7G019320 encodes GA3ox (gibberellin 3-beta-dioxygenase 1-like protein) with an SNP (single nucleotide polymorphism; A ◊ G) in an intron and a 20 bp deletion in the si-2 mutant. This fact strongly speculates that the GA3ox-mediated gibberellic acid (GA) signaling pathway could be involved in the si-2 mutant. It is well known that GAs have a significant impact on plant growth and development. In the si-2 mutant, a 20 bp deletion in the promoter may abolished the transcript levels of CsGy7G019320. Promoter analysis indicated that the 20 bp deleted promoter sequence of CsGy7G019320 may be considered as the AT-hook protein binding site. Although the involvement of the genes encoding GA3ox and AT-hook protein in GA signaling has been well identified, a 20 bp deletion in the promoter sequence of GA3ox in cucumber has not been revealed in other studies. Our data suggests that a mutation in the promoter sequence of CsGy7G019320 caused the si-2 mutant phenotype by suppression of GA3ox expression, possibly leading to decreased GA content in cucumber.

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Data availability

The gene (CsGy7G019320) and its promoter sequences obtained in this study have been deposited in the GenBank/NCBI database under the GenBank accession numbers; MZ647960 (promoter sequence of CsGy7G019320-WT), MZ647961 (promoter sequence of CsGy7G019320-SI), MZ670433 (gene sequence of CsGy7G019320-WT), and MZ670434 (gene sequence of CsGy7G019320-SI).

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Acknowledgements

We thank Yong Suk Chung for helpful comments on the manuscript. We would like to thank Editage (www.editage.co.kr) for English language editing. This work was supported by the cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01652901) of Rural Development Administration, Republic of Korea, and the Basic Science Research Program of the National Research Foundation (NRF) funded by the Republic of Korea’s Ministry of Education (Grant No. NRF 2020R1A2C108800).

Funding

This research was supported by the cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ016529012022) of Rural Development Administration, Republic of Korea, and the Basic Science Research Program of the National Research Foundation (NRF) funded by the Republic of Korea’s Ministry of Education (Grant No. NRF 2020R1A2C108800).

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Authors and Affiliations

  1. Plant Genomics Laboratory, Department of Bio-resource Engineering, College of Life Sciences, Sejong University, 209 Neungdong-ro, Gwanjing-gu, 05006, Seoul, Republic of Korea

    Mahdi Badri Anarjan, Shahida Begum & Sanghyeob Lee

  2. Breeding Research Institute, DONGOH SEED, 402, 17405, Icheon, Republic of Korea

    Ikhyun Bae

  3. Plant Engineering Research Institute, Sejong University, 05006, Seoul, Republic of Korea

    Sanghyeob Lee

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  1. Mahdi Badri Anarjan
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S. Lee planned experiments, analyzed the data and wrote the manuscript. S. Begum, and I. Bae conducted experiments and analyzed the data. M. Badri Anarjan conducted experiments, analyzed the data, and wrote the manuscript.

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Correspondence to Sanghyeob Lee.

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Author IB is employed by DONGHO Seed Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Communicated by Seon-In Yeom.

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Anarjan, M.B., Begum, S., Bae, I. et al. Mutation in the GA3ox gene governs short-internode characteristic in a korean cucumber inbred line. Hortic. Environ. Biotechnol. 64, 485–495 (2023). https://doi.org/10.1007/s13580-022-00496-6

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