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Biodegradation kinetics of microcystins-LR crude extract by Lysinibacillus boronitolerans strain CQ5

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Abstract

Purpose

As the most common variant of microcystins (MCs), microcystin-LR (MCLR) is a kind of toxins produced by some species of harmful cyanobacteria and more and more attention has been paid to it. Biodegradation has been extensively investigated and recognized to be a cost-efficient and environmentally benign method for MC clean-up. In order to further research the growth characteristics of strain and the biodegradation characteristics of MCLR, it is necessary to use the dynamic mathematical models as powerful and useful tools.

Methods

In this study, strain CQ5 was screened and identified by morphological observation, physiological and biochemical tests, and 16S rDNA sequence analysis. The kinetic models of cell growth and MCLR degradation were established with the Gompertz model and revised Monod kinetic model.

Results

The results showed that strain CQ5 had the closest phylogenetic similarity to Lysinibacillus boronitolerans (T-10a, AB199591) in the phylogenetic tree, with 99% bootstrap support. Strain CQ5 could utilize MCLR as the carbon and nitrogen source for growth. When the initial pH value was 7 and the inoculation amount was 3%, strain CQ5 grew well in MSM, in which the MCLR crude extract was used as the carbon and nitrogen source of strain CQ5. Within 244 h, the MCLR concentration changed from 14.12 to 1.57 μg/L and its degradation rate could reach 88.88%. The growth curve fitted with the Gompertz growth model (Nt = 1.3119 * exp(−0.1237 * exp(−6.6341t)), R2 > 0.99). The process of MCLR degradation agreed with the first-order reaction kinetic equation (lnS = 2.64764 − 0.01537t, R2 > 0.99). The linkage relationship between MCLR concentration, cell density, and MCLR degradation rate was consistent with the revised Monod equation (V = 0.342S, R2 > 0.97) at low substrate concentration, where Vmax/ Ks was 0.342. The dynamic relationship in which strain CQ5 degraded MCLR and used it as the carbon and nitrogen source to promote its own growth could be explained by the equation S = 14.12 e− 0.342 Nt (N = 1.08).

Conclusion

The growth of strain CQ5 and MCLR concentration in degradation system could be simulated and predicted by the dynamic mathematical models in this study. And the predicted results were very consistent. These results could provide theoretical reference for studying the mechanism of MCLR biodegradation and promote the engineering application of strain CQ5.

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Funding

This work was supported by the National Natural Science Foundation (No. 41571471), the Jiangsu Science and Technology Support Project (No. BE2016653), and the Major Science and Technology Projects for Water Pollution Control and Treatment (2017ZX07202-003/004).

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Authors and Affiliations

  1. School of Environmental and Safety Engineering, Changzhou University, Changzhou, 213164, China

    Rongyan Shen, Zehui Chen, Xiaona Dong, Hongchi Shen, Peng Su, Linqiang Mao & Wenyi Zhang

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  1. Rongyan Shen
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  2. Zehui Chen
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  3. Xiaona Dong
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  4. Hongchi Shen
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  5. Peng Su
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  6. Linqiang Mao
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  7. Wenyi Zhang
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Correspondence to Wenyi Zhang.

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Shen, R., Chen, Z., Dong, X. et al. Biodegradation kinetics of microcystins-LR crude extract by Lysinibacillus boronitolerans strain CQ5. Ann Microbiol 69, 1259–1266 (2019). https://doi.org/10.1007/s13213-019-01510-6

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