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ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

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Abstract

Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.

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Acknowledgment

The authors would like to express their sincere thanks to the National University of Malaysia (UKM) for the financial support under the grant, STGL-009-2008.

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Authors and Affiliations

  1. School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Fatemeh Mahmoodani, Masomeh Ghassem, Abdul Salam Babji & Salma Mohamad Yusop

  2. Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran

    Fatemeh Mahmoodani & Roya Khosrokhavar

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  1. Fatemeh Mahmoodani
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  2. Masomeh Ghassem
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  3. Abdul Salam Babji
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  4. Salma Mohamad Yusop
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Correspondence to Masomeh Ghassem.

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Mahmoodani, F., Ghassem, M., Babji, A.S. et al. ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate. J Food Sci Technol 51, 1847–1856 (2014). https://doi.org/10.1007/s13197-012-0742-8

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