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Exogenous application of glutathione enhanced growth, nutritional orchestration and physiochemical characteristics of Brassica oleracea L. under lead stress

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Abstract

A major obstacle to agricultural production and yield quality is heavy metal contamination of the soil and water, which leads to lower productivity and quality of crops. The situation has significantly worsened as a result of the growing population and subsequent rise in food consumption. The growth of nutrient-rich plants is hampered by lead (Pb) toxicity in the soil. Brassica oleracea L. (broccoli) is a prominent vegetable crop in the Brassicaceae family subjected to a number of biotic and abiotic stresses that dramatically lower crop yields. Seed priming is a novel, practicable, and cost-effective method that can improve various abiotic stress tolerances. Many plant metabolic activities depend on the antioxidant enzyme glutathione (GSH), which also chelates heavy metals. Keeping in view the stress mitigation potential of GSH, current research work was designed to inspect the beneficial role of seed priming with GSH on the growth, morphological and gas exchange attributes of broccoli seedlings under Pb stress. For this purpose, broccoli seeds were primed with 25, 50, and 75 µM L−1 GSH. Plant growth and photosynthetic activity were adversely affected by Pb stress. Furthermore, Pb stress enhanced proline levels along with reduced protein and phenol content. The application of GSH improved growth traits, total soluble proteins, chlorophyll content, mineral content, and gas exchange parameters. The involvement of GSH in reducing Pb concentrations was demonstrated by an improved metal tolerance index and lower Pb levels in broccoli plants. The results of the current study suggest that GSH can be used as a strategy to increase broccoli tolerance to Pb by enhancing nutrient uptake, growth and proline.

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Authors and Affiliations

  1. Institute of Botany, University of the Punjab, Lahore, Pakistan

    Maria Ahmad, Shakil Ahmed & Rehana Sardar

  2. SSG RO-II Department, University of the Punjab, Lahore, Pakistan

    Nasim Ahmad Yasin

  3. Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan

    Abdul Wahid

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  1. Maria Ahmad
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Maria Ahmad: Performed experiment, Data curation, Writing- Original draft preparation, Software. Prof. Dr. Shakil Ahmed: Conceptualization, Methodology, Supervision.  Dr. Nasim Ahmad Yasin: Statistical analysis, Editing, Validation, Dr. Rehana Sardar: Conceptualization, Visualization, Investigation, Supervision, Software. Prof. Dr. Abdul Wahid: Writing- Reviewing and Editing, Validation.

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Ahmad, M., Ahmed, S., Yasin, N.A. et al. Exogenous application of glutathione enhanced growth, nutritional orchestration and physiochemical characteristics of Brassica oleracea L. under lead stress. Physiol Mol Biol Plants 29, 1103–1116 (2023). https://doi.org/10.1007/s12298-023-01346-0

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