Abstract
Quantitative trait loci (QTLs) for Cu-tolerance were determined in wheat grown in control and Cu-treated soil in greenhouse. In addition, loci having an influence on the shoot Cu-, Fe-, Mn-and Zn-contents under non-stressed and Cu-stressed environments were mapped. One major QTL for Cu-tolerance was found on chromosome 5DL, while slighter effects were determined on the chromosomes 1AL, 2DS, 4AL, 5BL and 7DS. QTLs affecting the shoot Mn-and Zn-contents were found on the chromosomes 3BL and 3AL, respectively. The centromeric region on the chromosome 3B plays a role in the regulation of the shoot Fe-contents in the stressed plants. Under Cu-stress QTL affecting shoot Cu-content was found on chromosome 1BL, while on the chromosome 5AL a QTL influencing the Cu-accumulation ability of wheat from Cu-polluted soil was determined.
Similar content being viewed by others
Abbreviations
- ITMI:
-
International Triticeae Mapping Initiative
- LOD:
-
logarithmic odds
- LR:
-
likelihood ratio
- MT:
-
metallothionein
- QTL:
-
quantitative trait locus
- RIL:
-
recombinant inbred line
- SMA:
-
single marker ANOVA
References
Agrawal, V., Sarma, K.: Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica.-Biol. Plant. 50: 307–310, 2006.
Bálint, A.F., Kovács, G., Börner, A., Galiba, G., Sutka, J.: Substitution analysis of seedling stage copper tolerance in wheat.-Acta agron. hung. 51: 397–404, 2003.
Börner, A., Schumann, E., Fürste, A., Cöster, H., Leithold, B., Röder, M.S., Weber, W.E.: Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.).-Theor. appl. Genet. 105: 921–936, 2002.
Ganeva, G., Landjeva, S., Merakchijska, S.: Effects of chromosome substitutions on copper toxicity tolerance in wheat seedlings.-Biol. Plant. 47: 621–623, 2003.
Haley, C.S., Knott, S.A.: A simple regression method for mapping quantitative trait loci in line crosses using flanking markers.-Heredity 69: 315–324, 1992.
Hall, J.L.: Cellular mechanisms for heavy metal detoxification and tolerance.-J. exp. Bot. 53: 1–11, 2002.
Jefferies, S.P., Pallota, M.A., Paull, J.G., Karakousis, A., Kretschmer, J.M., Manning, S., Islam, A.K.M.R., Langridge, P., Chalmers, K.J.: Mapping and validation of chromosome regions conferring boron toxicity tolerance in wheat (Triticum aestivum).-Theor. appl. Genet. 101: 767–777, 2000.
Kawashima, I., Inokuchi, Y., Chino, M., Kimura, M., Shimizu, N.: Isolation of a gene for metallothionein-like protein from soybean.-Plant Cell Physiol. 32: 913–916, 1991.
Lanaras, T., Moustakas, M., Symenoidis, L., Diamantoglou, S., Karataglis, S.: Plant metal content, growth responses and some photosynthetic measurements on field-cultivated wheat growing on ore bodies enriched in Cu.-Physiol. Plant. 88: 307–314, 1993.
Lander, E.S., Botstein, D.: Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps.-Genetics 129: 185–199, 1989.
Macnair, M.R.: The genetics of metal tolerance in vascular plants.-New Phytol. 124: 541–559, 1993.
Manyowa, N.M., Miller, T.E.: The genetics of tolerance to high mineral contents in the tribe Triticeae — a review and update.-Euphytica 57: 175–185, 1991.
Marino, C.L., Nelson, J.C., Lu, Y.H., Sorrells, M.E., Leroy, P., Tuleen, N.A., Lopes, C.R., Hart, G.E.: Molecular genetics maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L. em. Thell.).-Genome 39: 359–366, 1996.
Moustakas, M., Ouzounidou, G., Symeonidis, L.: Field study of the effects of excess copper on wheat photosynthesis and productivity.-Soil Sci. Plant Nutr. 43: 531–539, 1997.
Nelson, J.C., Sorrells, M.E., Van Deynze, A.E., Lu, Y.H., Atkinson, M., Bernard, M., Leroy, P., Faris, J.D., Anderson, J.A.: Molecular mapping of wheat: major genes and rearrengements in homoeologous groups 4, 5, and 7.-Genetics 141: 721–731, 1995a.
Nelson, J.C., Van Deynze, A.E., Autrique, E., Sorrells, M.E., Lu, Y.H., Merlino, M., Atkinson, M., Leroy, P.: Molecular mapping of wheat: homoeologous group 2.-Genome 38: 516–523, 1995b.
Nelson, J.C., Van Deynze, A.E., Autrique, E., Sorrells, M.E., Lu, Y.H., Negre, S., Bernard, M., Leroy, P.: Molecular mapping of wheat: homoeologous group 3.-Genome 38: 524–533, 1995c.
Nian, H., Yang, Z.M., Ahn, S.J., Cheng, Z.J., Matsumoto, H.: A comparative study on the aluminium-and copper-induced organic acid exudation from wheat roots.-Physiol. Plant. 116: 328–335, 2002.
Ouzounidou, G., Ilias, I.: Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity.-Biol. Plant. 49: 223–228, 2005.
Salunkhe, D., Desphande, S.S.: Micronutritional efficiency in crop plants — a new challenge for cytogenetic research. In: Lelley, T. (ed.): Current Topics in Plant Cytogenetics Related to Plant Improvement. Pp. 91–101. WUV-Univ.-Verl., Wien 1991.
Schlegel, R., Cakmak, I., Torun, B., Eker, S., Köleli, N.: The effect of rye genetic information on zinc, copper, manganese and iron concentration of wheat shoots in zinc deficient soil.-Cereal Res. Commun. 25: 177–184, 1997.
Underwood, B.A., Smitasiri, S.: Micronutrient malnutrition: policies and programs for control and their implications.-Annu. Rev. Nutr. 19: 303–324, 1999.
Van Ooijen, J.W.: MapQTL® 5, Software for the calculation of QTL positions on genetic map.-www.kyazma.nl, 2003.
Welch, R.M.: Effects of nutrient deficiencies on seed production and quality.-Adv. Plant Nutr. 2: 205–247, 1986.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bálint, A.F., Röder, M.S., Hell, R. et al. Mapping of QTLs affecting copper tolerance and the Cu, Fe, Mn and Zn contents in the shoots of wheat seedlings. Biol Plant 51, 129–134 (2007). https://doi.org/10.1007/s10535-007-0025-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-007-0025-9