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Expression of Cell Cycle-Related Proteins, p16, p53 and p63 as Important Prognostic Markers in Gallbladder Adenocarcinoma

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Pathology & Oncology Research

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Abstract

Gallbladder cancer, the most common biliary tract malignancy, is a highly malignant neoplasm. In the present work, we have analyzed the significance of cell cycle-related proteins to predict prognosis and to provide guidance for optimal therapeutic decision-making in patients with gallbladder adenocarcinoma. The expressions of p16, p21, p27, p53, p63, cyclin D1, bcl-2 and bcl-6 were examined in a tissue microarray constructed from 96 cases of gallbladder adenocarcinoma by immunohistochemistry and correlated with clinicopathologic prognostic factors. Expression of p16 was correlated with a low pT stage, adenoma background and good prognosis. Cases with p63 expression showed a higher T stage, more frequent perineural invasion and poor prognosis when compared to cases without p63 expression. Over-expression of p53 or loss of p53 was associated with poor tumor differentiation, frequent distant metastasis and low disease-specific survival rate. The expressions of p21, p27, bcl-2, bcl-6 and cyclin D1 were not significant prognostic factors for gallbladder adenocarcinoma. These results indicate that p16, p63 and p53 can be used as prognostic markers in gallbladder adenocarcinoma; especially p53 and p63 as poor prognostic markers and p16 as a favorable prognostic marker.

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Acknowledgments

This work was supported by the Medical Research Funds from Kangbuk Samsung Hospital.

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Authors and Affiliations

  1. Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 108, Pyeong-dong, Jongro-gu, Seoul, 100-634, South Korea

    Kyungeun Kim, Dong-Hoon Kim, Seoung Wan Chae, Sung-Im Do, Hyun Joo Lee, Ji Hae Koo, Jung-Soo Pyo & Jin Hee Sohn

  2. Department of Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Jun-Ho Shin

  3. Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Hong Joo Kim

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  1. Kyungeun Kim
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  2. Dong-Hoon Kim
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Correspondence to Jin Hee Sohn.

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Kim, K., Kim, DH., Chae, S.W. et al. Expression of Cell Cycle-Related Proteins, p16, p53 and p63 as Important Prognostic Markers in Gallbladder Adenocarcinoma. Pathol. Oncol. Res. 20, 409–415 (2014). https://doi.org/10.1007/s12253-013-9710-5

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  • DOI: https://doi.org/10.1007/s12253-013-9710-5

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