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Expression of a Tumor Stem Cell Marker (Aldehyde Dehydrogenase 1-ALDH1) in Benign Epithelial Odontogenic Lesions

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Abstract

The morphological diversity and different biological behaviors of human lesions has been attributed to the presence of cells with stem cell (SC) characteristics. Among SC markers, ALDH1 has been used in studies investigating different neoplasms and high expression of this marker was associated with clinicopathological features and prognosis in some groups. The aim of this study was to analyze the presence and distribution of SCs based on the expression of ALDH1 in epithelial odontogenic cysts and tumors. The sample consisted of 80 cases (20 dentigerous cysts (DCs), 20 odontogenic keratocysts (OKCs), 20 ameloblastomas (AMs), and 20 adenomatoid odontogenic tumors (AOTs). An immunoreactivity score was obtained from the percentage of positive cells and intensity of immunostaining. A level of 5% (p < 0.05) was adopted for the statistical tests. Immunoexpression of ALDH1 was observed in cytoplasm and nucleus-cytoplasm. The median scores indicated significantly higher expression in OKCs and DCs compared to AMs (p < 0.0001) and AOTs (p < 0.0001). In the tumor stroma and cystic capsule, immunoreactivity was detected in all odontogenic cysts studied and in 85% and 90% of AMs and AOTs, respectively. The expression of ALDH1 suggests the presence of SCs in the odontogenic lesions studied. Epithelial immunoexpression was higher in odontogenic cysts than in odontogenic tumors.

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Funding

This study was financially supported by National Council for Scientific and Technological Development (CNPq). L.B.S is a Research Productivity fellow at CNPq.

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Authors and Affiliations

  1. Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Gustavo Alcântara da Trindade

  2. Federal University of Campina Grande, Patos, PB, Brazil

    Leorik Pereira da Silva

  3. Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Pedro Paulo de Andrade Santos

  4. Department of Oral Pathology, Federal University of Rio Grande do Norte, Av. Senador Salgado Filho, 1787, Lagoa Nova, Natal, RN, CEP 59056-000, Brazil

    Leão Pereira Pinto & Lélia Batista de Souza

Authors
  1. Gustavo Alcântara da Trindade
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  2. Leorik Pereira da Silva
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  3. Pedro Paulo de Andrade Santos
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  4. Leão Pereira Pinto
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  5. Lélia Batista de Souza
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Correspondence to Lélia Batista de Souza.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the Research Ethics Committee of UFRN. CEP-UFRN, Approval number 3.446.394.

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da Trindade, G.A., da Silva, L.P., de Andrade Santos, P.P. et al. Expression of a Tumor Stem Cell Marker (Aldehyde Dehydrogenase 1-ALDH1) in Benign Epithelial Odontogenic Lesions. Head and Neck Pathol 16, 785–791 (2022). https://doi.org/10.1007/s12105-022-01430-z

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  • DOI: https://doi.org/10.1007/s12105-022-01430-z

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