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Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions

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Abstract

Benign epithelial odontogenic lesions are great clinical importance entities that develop in the jaws from the tissues that form teeth. It has been shown that benign and malignant tumors are present in a large number of tumor stem cells, which has great implications in the development of these lesions. Oct-4 and CD44 have been demonstrated as important markers for tumoral stem cells. The aim of this study was investigate the presence of stem cell markers Oct-4 and CD44 in benign epithelial odontogenic lesions. Twenty odontogenic keratocysts (OKC), 20 ameloblastomas (AMB) of the solid/multicystic type and 20 adenomatoid odontogenic tumors (AOT) were retrospectively analyzed for immunohistochemical detection of Oct-4 and CD44 in their epithelial component. All cases were positive for the two markers, with the majority exhibiting a high expression. Analysis of the expression of Oct-4 revealed no statistically significant differences (p = 0.406) between the lesions studied. Regarding CD44, there was a significant difference between the cases of AMB and AOT in relation with OKC, with the latter presenting a greater labelling (p = 0.034). No statistically significant correlation between Oct-4 and CD44 was observed in the lesions. In our findings, the presence of stem cell-like phenotype at various sites of the epithelial component of the odontogenic lesions was identified, suggesting its possible participation in histogenesis and differentiation without, however, exerting influence on the aggressiveness of the lesions.

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Funding

This study was funded by National Council for Scientific and Technological Development (CNPq) (Grant no. 307539/2010-5)—Lélia Batista de Souza.

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Authors and Affiliations

  1. Post Graduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Eduardo Alonso Cruz Monroy

  2. Post Graduate Program in Oral Pathology, Department of Morphology, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Pedro Paulo de Andrade Santos

  3. Post Graduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Maria Luiza Diniz de Sousa Lopes

  4. Health Care Department, Universidad Autónoma Metropolitana, Xochimilco, Mexico

    Adalberto Mosqueda-Taylor

  5. Post Graduate Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Av. Senador Salgado Filho, 1787, Lagoa Nova, Natal, RN, CEP 59056-000, Brazil

    Leão Pereira Pinto & Lélia Batista de Souza

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  1. Eduardo Alonso Cruz Monroy
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  2. Pedro Paulo de Andrade Santos
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  3. Maria Luiza Diniz de Sousa Lopes
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  6. Lélia Batista de Souza
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Correspondence to Lélia Batista de Souza.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Monroy, E.A.C., de Andrade Santos, P.P., de Sousa Lopes, M.L.D. et al. Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions. Histochem Cell Biol 150, 371–377 (2018). https://doi.org/10.1007/s00418-018-1692-7

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  • DOI: https://doi.org/10.1007/s00418-018-1692-7

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