Abstract
In the present investigation, influence of exogenous phenolic acids on antioxidative defense system of salt stressed wheat seedlings was explored at the seventh day of growth. Electrical conductivity of 10 dS/m was used for imposing salt stress in two wheat cultivars showing contrasting behavior for salt tolerance. For observing stress mitigating effects of various phenolic acids, 20 ppm of ferulic, 10 ppm of caffeic, 10 ppm of p-coumaric, 5 ppm of salicylic, and 15 ppm of sinapic acids were selected for biochemical studies. Imposition of salinity stress reduced membrane stability as depicted by electrolyte leakage and reduction was more in sensitive cultivar HD2329 which was well correlated with its higher ROS accumulation in terms of H2O2 content and lipid peroxidation as MDA content. Exogenous application of phenolic acids reduced electrolyte leakage in NaCl-stressed seedlings of both the cultivars and maximum decrease was observed in the presence of sinapic acid, followed by caffeic, salicylic, ferulic, and p-coumaric acids. When phenolic acids were applied to salt stressed wheat seedlings, malondialdehyde content either decreased or remained unaffected in the shoots of both the cultivars, whereas hydrogen peroxide (H2O2) decreased in the roots and shoots of both cultivars maximally by caffeic and salicylic acids. Hydroxyl radical scavenging capacity of salt stressed seedlings increased to the maximum extent by the use of caffeic and sinapic acids. Catalase (CAT) and peroxidase activities were upregulated in the stressed shoots of salt-tolerant cultivar by the exogenous use of caffeic and sinapic acids. In comparison to stress, ascorbate peroxidase (APX) activity was also upregulated in stressed seedlings of both cultivars by exogenous use of caffeic and sinapic acids. In stressed seedlings of salt-sensitive cultivar, monodehydroascorbate reductase activity increased by exogenous use of caffeic, p-coumaric, salicylic, and sinapic acids. In roots of Kharchia local, use of ferulic, p-coumaric, and caffeic acids resulted into upregulation of glutathione reductase activity, whereas in salt-sensitive cultivar, only caffeic acid caused upregulation of this enzyme. Proline (Pro) content increased in HD2329 on addition of different exogenous phenolic acids in the medium, whereas in Kharchia local, addition of sinapic acid enhanced pro content. Glycine betaine (GB) content was increased by use of different phenolic acids in the stressed roots of Kharchia local. On the other hand, exogenous application of sinapic acid led to enhanced GB content in salt-sensitive cultivar. Based upon the fine regulation of CAT and APX activities and hydroxyl radical scavenging activity in relation to H2O2 content and electrolyte leakage, caffeic and sinapic acids may be regarded as the most efficient among the different phenolic acids in averting ROS-accrued oxidative damage in salt stressed wheat seedlings.
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References
Aghdam MS, Mohammadkhani N (2014) Enhancement of chilling stress tolerance of tomato fruit by postharvest brassinolide treatment. Food Bioprocess Tech 7:909–914
Agriculture year book (2016) http://icfa.org.in/YB-2016.pdf. Accessed 10 Apr 2017
Alexieva V, Sergiev I, Mapelli S, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344
Arshi A, Ahmad A, Aref IM, Iqbal M (2010) Effect of calcium against salinity-induced inhibition in growth, ion accumulation and proline contents in (Cichorium intybus L.). J Environ Biol 31:939–944
Azooz MM (2009) Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. Int J Agric Biol 11:343–350
Bates LS, Waldan RP, Teare LD (1973) Rapid determination of free proline under water stress studies. Plant Soil 39:205–207
Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcic DZ, Mimica-Dukic NM (2009) Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. J Agric Food Chem 57:9268–9273
Bhardwaj R, Sharma A, Sharma H, Srivastva P (2015) Role of gallic acid pre-treatment in inducing the antioxidant response of two wheat cultivars differing in drought tolerance. Ind J Agric Biochem 28:155–165
Chakraborty K, Bishi SK, Goswami N, Singh AL, Zela PV (2016) Differential fine-regulation of enzyme driven ROS detoxification network imparts salt tolerance in contrasting peanut genotypes. Environ Exp Bot 128:79–90
Chance B, Maehly AC (1955) Assay of catalase and peroxidases. Methods Enzymol 11:764–775
Das K, Roychoudhury A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci. doi:10.3389/fenvs.2014.00053
Din J, Khan US, Ali I, Gurmani AR (2011) Physiological and agronomic response of canola varieties to drought stress. J Animal Plant Sci 21:78–82
Elhamid EM, Sadak M, Tawfik M (2014) Alleviation of adverse effects of salt stress in wheat cultivars by foliar treatment with antioxidant changes in some biochemical aspects, lipid peroxidation, antioxidant enzymes and amino acid contents. Agric Sci 5:1269–1280
Ellouzi H, Hamed KB, Cela J, Munne-Bosch S, Abdelly C (2011) Early effects of salt stress on the physiological and oxidative status of Cakile maritima (halophyte) and Arabidopsis thaliana (glycophyte). Physiol Plant 142:128–143
Esterbauer H, Grill D (1978) Seasonal variation of glutathione and glutathione reductase in needless of Picea abies. Plant Physiol 61:119–121
Farooq M, Hussain M, Wakeel A, Siddique K (2015) Salt stress in maize: effects, resistance mechanisms and management. A review. Agron Sustain Dev 35:461–481
Fayez KA, Bazaid SA (2014) Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. J Saudi Soc Agric Sci 13:45–55
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
Grieve CM, Grattan SR (1983) Rapid assay for the determination of water soluble quaternary ammonium compounds. Plant Soil 70:303–307
Hasanuzzaman M, Hossain MA, Fujita M (2012) Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings. Plant Biotechnol Rep 5:353–365
Heath R, Packer L (1968) Photoperoxidation in isolated chloroplast I Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in the regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395
Ismail A, Takeda S, Peter N (2015) Life and death under salt stress: same players, different timings? J Exp Bot. doi:10.1093/jxb/eru159
Jamil A, Riaz S, Ashraf M, Foodlad MR (2011) Gene expression profiling of plants under salt stress. Crit Rev Plant Sci 30:435–458
Jayakannan M, Bose J, Babourina O, Rengel Z, Shabala S (2013) Salicylic acid improves salinity tolerance in arabidopsis by restoring membrane potential and preventing salt induced K+ loss via a GORK channel. J Exp Bot 10:1093–1096
Jiang X, Li H, Song X (2016) Seed priming with melatonin effects on seed germination and seedling growth in maize under salinity stress. Pak J Bot 48:1345–1352
Kang G, Li G, Xu W, Peng X, Han Q, Zhu Y (2012) Proteomics reveals the effects of salicylic acid on growth and tolerance to subsequent drought stress in wheat. J Proteome Res 11:6066–6079
Kapoor D, Sharma R, Handa N, Kaur H, Rattan A, Yadav P (2015) Redox homeostasis in plants under abiotic stress: role of electron carries, energy metabolism mediators and proteinaceous thiols. Front Environ Sci. doi:10.3389/fenvs.2015.00013
Karamac M, Kosinska A, Pegg RB (2005) Comparison of radical-scavenging activities for selected phenolic acids. Pol J Food Nutr Sci 14:165–170
Klein AM, Mazutis L, Akartuna I, Tallapragada N, Veres A, Li V, Peshkin L, Weitz DA, Kirschner MW (2015) Droplet barcoding for single cell transcriptomics and its application to embryonic stem cells. Cell 161:1187–1201
Koca H, Bor M, Ozdemir F, Turkan I (2007) The effect of salt stress on lipid peroxidation antioxidative enzymes and proline content of sesame cultivars. Environ Exp Bot 60:344–351
Law MY, Charles SA, Halliwell B (1983) Glutathione and ascorbic acid in spinach (Spinacea oleracea) chloroplast. J Biochem 47:378–382
Lowry OH, Rosebrough NT, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275
Lutts S, Kinet JM, Bouharmont J (1996) NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann Bot 78:389–398
Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:169–174
Mathew S, Abraham TE, Zakaria Z (2015) Reactivity of phenolic compounds towards free radicals under in vitro conditions. J Food Sci Technol 52:5790–5798
Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud 15:523–530
Mittler R (2017) ROS are good. Trends Plant Sci. doi:10.1016/j.tplants.2016.08.002
Miura K, Tada Y (2014) Regulation of water, salinity and cold stress responses by salicylic acid. Front Plant Sci. doi:10.3389/fpls.2014.00004
Moussa HR, Abdel-Aziz SM (2008) Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Aust J Crop Sci 1:31–36
Nahar K, Hasanuzzaman M, Mahabub AM, Fujita M (2015) Exogenous glutathione confers high temperature stress tolerance in mung bean (Vigna radiata L.) by modulating antioxidant defense and methylglyoxal detoxification system. Environ Exp Bot 112:44–54
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Noreen S, Ashraf M, Hussain M, Jamil A (2009) Exogenous application of salicylic acid enhances antioxidative capacity in salt stressed sunflower (Helianthus annuus L.). Plants J Pak J Bot 41:473–479
Oidaira H, Sano SQ, Koshiba T, Ushimaru T (2000) Enhancement of antioxidative enzyme activities in chilled rice seedlings. J Plant Physiol 156:811–813
Reddy PS, Jogeshwar G, Rasineni G, Maheshwari M, Reddy AR, Varshney RK, Kishor PV (2015) Proline over-accumulation alleviated salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum [Sorghum bicolor (L.) Moench]. Plant Physiol Biochem 12:104–113
Schmidt M, Horstmann S, Colli L, Danaher M, Karl S, Emanuele Z, Arendt E (2016) Impact of fungal contamination of wheat on grain quality criteria. J Cereal Sci 69:95–103
Sergio L, Paola AD, Cantore V, Pieralice M, Cascarano NA, Bianco V, Venere D (2012) Effect of salt stress on growth parameters, enzymatic antioxidant system, and lipid peroxidation in wild chicory (Cichorium intybus L.). Acta Physiol Plant 34:2349–2358
Sewelam N, Kazan K, Schenk MP (2016) Global plant stress signaling: reactive oxygen species at the cross road. Front Plant Sci. doi:10.3389/fpls.2016.00187
Shannon LM, Kay E, Lew JK (1966) Peroxidase isoenzyme from horseradish roots isolation and physical properties. J Biol Chem 241:2166–2172
Sharma P, Jha AB, Dubey RS, Pessaraki M (2012) Reactive oxygen species, oxidative damage and antioxidative defense mechanism in plants under stressful conditions. J Bot 3:26–32
Sharma A, Bhardwaj RD, Gupta AK (2015) Ferulic acid: a novel inducer of antioxidant enzymes in wheat (Triticum aestivum L.). Seedlings Cereal Res Commun 43:394–402
Shrivastva P, Kumar R (2014) Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J Biol Sci 22:123–131
Singh N, Bhardwaj RD (2016) Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants. Biologia. doi:10.1515/biolog-2016-0050
Sofo A, Scopa A, Nuzzaci M, Vitti A (2015) Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. Int J Mol Sci 16:13561–13578
Tari I, Csiszar J, Hovarth E, Poor P, Takacs Z, Szepsi A (2015) The alleviation of adverse effects of salt stress in tomato plants by salicylic acid shows a time and organ specific antioxidant responses. Acta Biologica Cracoviensia 57:21–30
Tuna AL, Kaya C, Altunlu H, Ashraf M (2013) Mitigation effects of non-enzymatic antioxidants in maize (Zea mays L.) plants under salinity stress. Aust J Crop Sci 7:1181–1188
Venkatesh J, Park SW (2014) Role of l-ascorbate in alleviating abiotic stresses in crop plants. Bot Stud 55:38
Wan YY, Zhang Y, Zhang L, Zhou ZQ, Li X, Shi Q, Wang XJ, Bai JG (2015) Caffeic acid protects cucumber against chilling stress by regulating antioxidant enzyme activity and proline and soluble sugar contents. Acta Physiol Plant 37:1706–1715
Wang J, Islam F, Gill AR, Yang C, Ali B, Zhou W (2016) Salicylic acid mediates antioxidative defense system and ABA pathway related gene expression in Orzya sativa against quinclorac toxicity. Ecotoxi Environ Safety 133:146–156
Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Golezani KG (2012) Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J 5:60–67
Yu ZM, Kang B, He XW, Lü SL, Bai YH, Ding WN, Chen M, Cho HT, Wu P (2012) Root hair-specific expansions modulate root hair elongation in rice. Plant J 66:725–734
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The corresponding author is highly thankful to University Grants Commission, New Delhi vide F. no. 42-655/2013 (SR) for funding this research.
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Kaur, H., Bhardwaj, R.D. & Grewal, S.K. Mitigation of salinity-induced oxidative damage in wheat (Triticum aestivum L.) seedlings by exogenous application of phenolic acids. Acta Physiol Plant 39, 221 (2017). https://doi.org/10.1007/s11738-017-2521-7
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DOI: https://doi.org/10.1007/s11738-017-2521-7