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Antioxidant system in programmed cell death of sycamore (Acer pseudoplatanus L.) cultured cells

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Abstract

Reactive oxygen species (ROS) have pleiotropic effects in plants. ROS can lead to cellular damage and death or play key roles in control and regulation of biological processes, such as programmed cell death (PCD). This dual role of ROS, as toxic or signalling molecules, is possible because plant antioxidant system (AS) is able to achieve a tight control over ROS cellular levels, balancing properly their production and scavenging. AS response in plant PCD has been clearly described only in the hypersensitive response in incompatible plant–pathogen interactions and in the senescence process and has not been completely unravelled. In sycamore (Acer pseudoplatanus L.) cultured cells PCD can be induced by Fusicoccin (Fc), Tunicamycin (Tu), and Brefeldin A (Ba). These chemicals induce comparable PCD time course and extent, while H2O2 production is detectable only in Fc- and, to a lesser extent, in Ba-treated cells. In this paper the AS has been investigated during PCD of sycamore cells, measuring the effects of the three inducers on the cellular levels of non-enzymatic and enzymatic antioxidants. Results show that the AS behaviour is different in the PCD induced by the three chemicals. In Fc-treated cells AS is mainly devoted to decrease the concentration of toxic intracellular H2O2 levels. On the contrary, in cells treated with Tu and Ba, the cell redox state is shifted to a more reduced state and the enzymatic AS is partially down-regulated, allowing ROS to act as signalling molecules.

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Abbreviations

APX:

Ascorbate peroxidase

AS:

Antioxidant system

ASA:

Ascorbic acid

Ba:

Brefeldin A

CAT:

Catalase

DHA:

Dehydroascorbic acid

DR:

Dehydroascorbate reductase

Fc:

Fusicoccin

GPX:

Glutathione peroxidase

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

MR:

Monodehydroascorbate reductase

PCD:

Programmed cell death

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Tu:

Tunicamycin

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Acknowledgments

The authors wish to thank Riccardo Rizzetto for his practical support. The authors wish also to thank Prof. T. Koshiba for APX antibody, Prof. T. Ushimaru for DR and MnSOD antibodies, and Prof. S. Kanematsu for CuZnSOD antibody.

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Authors and Affiliations

  1. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy

    Nicla Contran, Mariagrazia Tonelli, Paolo Crosti & Massimo Malerba

  2. Department of Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1, 20126, Milan, Italy

    Raffaella Cerana

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  1. Nicla Contran
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  2. Mariagrazia Tonelli
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  3. Paolo Crosti
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  4. Raffaella Cerana
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  5. Massimo Malerba
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Corresponding author

Correspondence to Massimo Malerba.

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Communicated by G. Bartosz.

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Contran, N., Tonelli, M., Crosti, P. et al. Antioxidant system in programmed cell death of sycamore (Acer pseudoplatanus L.) cultured cells. Acta Physiol Plant 34, 617–629 (2012). https://doi.org/10.1007/s11738-011-0862-1

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  • DOI: https://doi.org/10.1007/s11738-011-0862-1

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