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Cyclosporin A inhibits programmed cell death and cytochrome c release induced by fusicoccin in sycamore cells

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Summary.

Programmed cell death plays a vital role in normal plant development, response to environmental stresses, and defense against pathogen attack. Different types of programmed cell death occur in plants and the involvement of mitochondria is still under investigation. In sycamore (Acer pseudoplatanus L.) cultured cells, the phytotoxin fusicoccin induces cell death that shows apoptotic features, including chromatin condensation, DNA fragmentation, and release of cytochrome c from mitochondria. In this work, we show that cyclosporin A, an inhibitor of the permeability transition pore of animal mitochondria, inhibits the cell death, DNA fragmentation, and cytochrome c release induced by fusicoccin. In addition, we show that fusicoccin induces a change in the shape of mitochondria which is not prevented by cyclosporin A. These results suggest that the release of cytochrome c induced by fusicoccin occurs through a cyclosporin A-sensitive system that is similar to the permeability transition pore of animal mitochondria and they make it tempting to speculate that this release may be involved in the phytotoxin-induced programmed cell death of sycamore cells.

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Abbreviations

BiP:

binding protein

CsA:

cyclosporin A

ER:

endoplasmic reticulum

FC:

fusicoccin

NO:

nitric oxide

PCD:

programmed cell death

PTP:

permeability transition pore

TUNEL:

terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling

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Authors and Affiliations

  1. Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Milan

    N. Contran, P. Crosti & M. Malerba

  2. Dipartimento di Scienze dell’Ambiente e del Territorio, Università degli Studi di Milano-Bicocca, Milan

    N. Contran & R. Cerana

Authors
  1. N. Contran
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  2. R. Cerana
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  3. P. Crosti
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  4. M. Malerba
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Correspondence to M. Malerba.

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Correspondence and reprints: Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.

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Contran, N., Cerana, R., Crosti, P. et al. Cyclosporin A inhibits programmed cell death and cytochrome c release induced by fusicoccin in sycamore cells. Protoplasma 231, 193–199 (2007). https://doi.org/10.1007/s00709-007-0250-2

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  • DOI: https://doi.org/10.1007/s00709-007-0250-2

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