Summary
The recent identification ofDAD (defender against apoptotic death) gene in plants suggests that the N-linked glycosylation of proteins could be an important control point of plant programmed cell death. In this paper we describe the effects of Tunicamycin, an inhibitor of N-linked protein glycosylation, and Brefeldin A, an inhibitor of protein trafficking from the Golgi apparatus, on sycamore (Acer pseudoplatanus L.) cell cultures. These two chemicals proved able to induce a strong acceleration of the cell death; changes in cell and nucleus morphology; an increase in DNA fragmentation, detectable by a specific immunological reaction; and the presence of oligonucleosomal-size fragments (laddering) in DNA gel electrophoresis. Moreover, Brefeldin A, but not Tunicamycin, strongly stimulated the production of hydrogen peroxide. These results indicate that also in plants chemicals interfering with the activities of endoplasmic reticulum and of Golgi apparatus strongly induce a form of programmed cell death showing apoptotic features.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Apte SS, Mattei MG, Scidin MF, Olsen BR (1995) The highly conserved defender against the death 1 (DAD1) gene maps to human chromosome 14qll-ql2 and mouse chromosome 14 and has plant and nematode homologs. FEBS Lett 363: 304–306
Aravind L, Dixit VM, Koonin EV (1999) The domains of death: evolution of the apoptosis machinery. Trends Biochem Sci 24: 47–53
Beaver JP, Stoneman CL (1999) Exposure of N-acetylglucosamine decreases early in dexamethasone-induced apoptosis in thymocytes, demonstrated by flow cytometry using wheat germ agglutinin and pokeweed mitogen. Immunol Cell Biol 77: 224–235
Ceriotti A, Duranti M, Bollini R (1998) Effects of N-glycosylation on the folding and structure of plant protein. J Exp Bot 49: 1091–1103
Danon A, Delorme V, Mailhac N, Gallois P (2000) Plant programmed cell death: a common way to die. Plant Physiol Biochem 38: 647–655
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Desagher S, Martinou JC (2000) Mitochondria as the central control point of apoptosis. Trends Cell Biol 10: 369–377
Drieu A, Carlberg M, Wang M, Larsson O (1997) Inhibition of N-linked glycosylation using Tunicamycin causes cell death in malignant cells: role of down-regulation of the insulin like growth factor 1 receptor in induction of apoptosis. Cancer Res 57: 543–546
Driouich A, Gönnet P, Makkie M, Laine A-C, Faye L (1989) The role of high mannose and complex asparagine-linked glycans in the secretion and stability of glycoproteins. Planta 180: 96–104
Faye L, Chrispeels MJ (1985) Characterization of N-linked oligosaccharides by affmoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases. Anal Biochem 149: 218–224
Gallois P, Makishima T, Hecht V, Despres B, Laudié M, Nishimoto T, Cooke R (1997) AnArabidopsis thaliana cDNA complementing a Hamster apoptosis suppressor mutant. Plant J 11: 1325–1331
Greenberg JT (1996) Programmed cell death: a way of life for plants. Proc Natl Acad Sci USA 93:12094–12097
Kelleher DJ, Gilmore R (1997) DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyl-transferase. Proc Natl Acad Sci USA 9: 4994–4999
Jabs T (1999) Reactive oxygen intermediates as mediators of programmed cell death in plants and animals. Biochem Pharmacol 57:231–245
Jacobson MD, Weil M, Raff MC (1997) Programmed cell death in animal development. Cell 88: 347–354
Lanubile R, Piro G, Dalessandro G (1997) Effect of Brefeldin A on the synthesis and transport of cell wall polysaccharides and protein in pea root seedlings. J Exp Bot 48:1925–1933
Malerba M, Crosti P, Armocida D, Bianchetti R (1995) Activation of ethylene production inAcer pseudoplatanus L. cultured cells by fusicoccin. J Plant Physiol 145: 93–100
Murphy TM, Huerta AJ (1990) Hydrogen peroxide format: cultured rose cells in response to UV-C radiation Physiol 78: 247–253
Nakashima T, Sekiguchi T, Kuraoka A, Fukushima K, Shib Komiyama S, Nishimoto T (1993) Molecular cloning of a h cDNA encoding a novel protein, DAD1, whose defect apoptotic cell death in hamster BHK21 cells. Mol Cell Bi 6367–6374
Nicholson DW, Thornberry NA (1997) Caspases: killer pro1 Trends Biochem Sci 22:299–305
Okushima Y, Koizumi N, Sano H (1999) Glycosylation a adequate processing is critical for protein secretion in to BY2 cells. J Plant Physiol 154: 623–627
Orzaez D, Granell A (1997) The plant homologue of the def against apoptotic death gene is down-regulated during senes of flower petals. FEBS Letts 404: 275–278
Pennell RI, Lamb C (1997) Programmed cell death in plants. Cell 9:1157–1168
Pontier D, Gan S, Amasino RM, Roby D, Lam E (1999) Marks hypersensitive response and senescence show distinct patte expression. Plant Mol Biol 39:1243–1255
Raff W (1998) Cell suicide for beginners. Nature 396:119–12: Schwartz LM, Smith SW, Jones MEE, Osborne BA (1993) 1 programmed cell death occur via apoptosis? Proc Natl Ac USA 90: 980–984
Scitz WR (1978) Chemioluminescence detection of enzymicall erated peroxide. Methods Enzymol 57: 445–461
Shao RG, Shimizu T, Pommier Y (1996) Brefeldin A is a l inducer of apoptosis in human cancer cells independently c Exp Cell Res 227:190–196
Shneyour A, Avron M (1970) High biological activity in chlore fromEuglena gracilis prepared with a new gas pressure d FEBS Lett 8:164–165
Singh M, Krikorian AD (1980) White’s standard nutrient sol Ann Bot 47:133–139
Staehelin LA, Driouich A (1997) Brefeldin A effects in plants. Physiol 114: 401–403
Sugimoto A, Hozack RR, Nakashima T, Nishimoto T, Rothm (1995)dadl, an endogenous programmed cell death suppresCaenorhabditis elegans and vertebrate. EMBO J 14:4434-’
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Crosti, P., Malerba, M. & Bianchetti, R. Tunicamycin and brefeldin a induce in plant cells a programmed cell death showing apoptotic features. Protoplasma 216, 31–38 (2001). https://doi.org/10.1007/BF02680128
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02680128