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Lipopolysaccharide-induced Trigeminal Ganglion Nerve Fiber Damage is Associated with Autophagy Inhibition

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Abstract

Objective

This study aimed to determine whether lipopolysaccharide (LPS) induces the loss of corneal nerve fibers in cultured trigeminal ganglion (TG) cells, and the underlying mechanism of LPS-induced TG neurite damage.

Methods

TG neurons were isolated from C57BL/6 mice, and the cell viability and purity were maintained for up to 7 days. Then, they were treated with LPS (1 µg/mL) or the autophagy regulator (autophibib and rapamycin) alone or in combination for 48 h, and the length of neurites in TG cells was examined by the immunofluorescence staining of the neuron-specific protein β3-tubulin. Afterwards, the molecular mechanisms by which LPS induces TG neuron damage were explored.

Results

The immunofluorescence staining revealed that the average length of neurites in TG cells significantly decreased after LPS treatment. Importantly, LPS induced the impairment of autophagic flux in TG cells, which was evidenced by the increase in the accumulation of LC3 and p62 proteins. The pharmacological inhibition of autophagy by autophinib dramatically reduced the length of TG neurites. However, the rapamycin-induced activation of autophagy significantly lessened the effect of LPS on the degeneration of TG neurites.

Conclusion

LPS-induced autophagy inhibition contributes to the loss of TG neurites.

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Authors and Affiliations

  1. Refractive Surgery Center, Shaanxi Eye Hospital, Xi’an People’s Hospital (Xi’an Fourth Hospital), Affiliated Guangren Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, China

    Yong Li, Jing Li, Sheng-sheng Wei & Jing Du

Authors
  1. Yong Li
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  2. Jing Li
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  3. Sheng-sheng Wei
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  4. Jing Du
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Corresponding author

Correspondence to Jing Du.

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Conflict of Interest Statement

No potential conflicts of interest were disclosed.

This work was supported by the Key Research and Development Program of Shaanxi Provice (No. 2023-YBSF-586), Natural Science Basic Research Plan of Shaanxi Province of China (No. 2017JM8043), the Health Research Project of Shaanxi Province (No. 2020yb11), and the Science Research of Xi’an Fourth Hospital of Shaanxi Province of China (No. FZ-4).

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Li, Y., Li, J., Wei, Ss. et al. Lipopolysaccharide-induced Trigeminal Ganglion Nerve Fiber Damage is Associated with Autophagy Inhibition. CURR MED SCI 43, 489–495 (2023). https://doi.org/10.1007/s11596-023-2739-0

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  • DOI: https://doi.org/10.1007/s11596-023-2739-0

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