Abstract
Obesity, an extremely important factor in feline clinical practice, is estimated to affect up to one third of the feline population. Moreover, it can trigger chronic inflammation, which could predispose to oxidative stress by increasing reactive oxygen species, thereby generating potentially irreversible cellular damage. This study analyzed hematological, biochemical and oxidative stress profiles at various degrees of feline obesity. Forty-five cats were selected and divided into three groups: control (n = 17), overweight (n = 13) and obese (n = 15), after clinical and laboratory evaluation and body condition score. Biochemical and oxidative stress analyses were performed using a photocolorimeter and hematological analyses were performed in a veterinary cell counter. Obese cats showed increased mean corpuscular volume (MCV), red cell distribution width (RDW), HDL cholesterol and triglycerides and decreased activity of gamma-glutamyl transferase (GGT) than control cats, although within the reference ranges for the species. As for oxidative stress, obese cats showed higher total antioxidant capacity (TAC), by the inhibition of 2,2’-Azino-Bis-3-Ethylbenzthiazoline-6-Sulfonic Acid (ABTS), inhibition of ABTS associated with horseradish peroxidase (ABTS + HRP), cupric ion reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP) methods, while overweight cats had a higher TAC-ABTS + HRP and TAC-FRAP than control cats. We conclude that the conditions of natural obesity and overweight in the feline species alter its hematological, biochemical and oxidative stress parameters.
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Data Availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
To São Paulo Research Foundation (FAPESP, Proc. 2018/18220-0) and CAPES (AUXPE 0255/2021, n. 88881.638964/2021-01) for funding this research, and FAPESP for the scholarship granted (2018/19683-4).
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This work was supported by São Paulo Research Foundation (FAPESP, Proc. 2018/18220-0) and CAPES (AUXPE 0255/2021, n. 88881.638964/2021-01). Author T.O.M. has received a research scholarship from FAPESP (2018/19683-4).
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The study conception and design were performed by Beatriz Perez Floriano, Marcel Gambin Marques and Breno Fernando Martins de Almeida. Animal selection, material preparation and sample collection were performed by Tainara de Oliveira Martins, Rebecca Cápera Ramos, Geovana Possidonio, Vinicius Aquiles Zamboni, Marcel Gambin Marques and Breno Fernando Martins de Almeida. Laboratory analysis were performed by Tainara de Oliveira Martins, Rebecca Cápera Ramos, Maria Rachel Melo Bosculo, Paula Lima Oliveira, Leticia Ramos Costa, Vinicius Aquiles Zamboni and Breno Fernando Martins de Almeida. The first draft of the manuscript was written by Tainara de Oliveira Martins and Rebecca Cápera Ramos and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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The experiment was conducted according to the ethical principles of the Animal Research Ethics Committee of the University Center of the Integrated Faculties of Ourinhos (Protocol no. 007/2019). The participation of each feline was authorized by its owner, who signed a free and informed consent form.
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Martins, T.d., Ramos, R.C., Possidonio, G. et al. Feline obesity causes hematological and biochemical changes and oxidative stress – a pilot study. Vet Res Commun 47, 167–177 (2023). https://doi.org/10.1007/s11259-022-09940-5
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DOI: https://doi.org/10.1007/s11259-022-09940-5