Abstract
Lipemia is one of the main causes of rejection of samples in veterinary clinical laboratories, interfering in biochemical determinations performed through spectrophotometry. However, it is not adequately known whether such interference causes clinical errors of interpretation in routine canine biochemical determinations, which is especially important when considering that lipemia cannot always be avoided in routine veterinary practice. This study assessed postprandial lipemia of healthy dogs fed with commercial feed, as well as the effect of lipemia induced in vitro by the addition of commercial lipid solution. Twenty healthy dogs were selected, for induction of postprandial lipemia, and the peak lipemic time (3 h after feeding) was compared with two non-lipemic times (12 h of fasting), 1 day before and 1 day after the induction of lipemia, respectively. Lipid solution was added to non-lipemic sera simulating triglyceride concentrations similar to the minimum, medium, and maximum values obtained at the lipemic time in vivo. Biochemical analyses were performed in semi-automated spectrophotometer using a set of commercial reagents. Variables were tested for normality and differences between the groups were tested using paired t test or Wilcoxon and ANOVA or Friedman tests. Both in vivo and in vitro lipemia caused significant biochemical changes in routine tests in healthy dogs, varying according to the analyte tested. Results from lipemic samples should be interpreted with caution for some determinations, whereas in some analyses, the need for fasting is dispensable.
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References
Alleman AR (1990) The effects of hemolysis and lipemia on serum biochemical constituents. Vet Med 85:1272–1284
Bauer JE (1996) Comparative lipid and lipoprotein metabolism. Vet Clin Pathol 25:49–56
Bauer JE (2004) Lipoprotein-mediated transport of dietary and synthesized lipids and lipid abnormalities of dogs and cats. J Am Vet Med Assoc 224:668–675
Bush BM (1991) Interpretation of laboratory results for small animal clinicians, 1st edn. Blackwell Scientific Publications, London
Chikazawa S, Hori Y, Kanai K et al (2013) Factors influencing measurement of serum iron concentration in dogs: diurnal variation and hyperferritinemia. J Vet Med Sci 75:1615–1618. https://doi.org/10.1292/JVMS.13-0286
CLSI (2012) Hemolysis, icterus, and lipemia/turbidity indices as indicators of interference in clinical laboratory analysis; approved guideline. CLSI C56-A document.
Glick MR, Ryder KW, Glick SJ, Woods JR (1989) Unreliable visual estimation of the incidence and amount of turbidity, hemolysis, and icterus in serum from hospitalized patients. Clin Chem 35:837–839
González FHD, Carvalho V, Möller VA, Duarte FR (2001) Perfil bioquímico sangüíneo de cães e gatos na cidade de Porto Alegre, Rio Grande do Sul, Brasil. Arq da Fac Vet UFRGS 29:1–6
Jacobs RM, Lumsden JH, Grift E (1992) Effects of bilirubinemia, hemolysis, and lipemia on clinical chemistry analytes in bovine, canine, equine, and feline sera. Can Vet J 33:605–608
Johnson MC (2005) Hyperlipidemia disorders in dogs. Compendium:361–370
Kaneko JJ, Harvey JW, Bruss ML (2008) Clinical biochemistry of domestic animals, 6th edn. Academic Press, London
Kazmierczak SC (2013) Hemolysis, lipemia, and high bilirubin: effect on laboratory tests. In: Dasgupta A, Sepulveda JL (eds) . Elsevier, Amsterdam, pp 53–62
Laflamme D (1997) Development and validation of a body condition score system for dogs. Canine Pract 22:10–15
Martínez-Subiela S, Cerón JJ (2005) Effects of hemolysis, lipemia, hyperbilirrubinemia, and anticoagulants in canine C-reactive protein, serum amyloid A, and ceruloplasmin assays. Can Vet J 46:625–629
Moreno P, Ginel PJ (1999) Effects of haemolysis, lipaemia and bilirubinaemia on prothrombin time, activated partial thromboplastin time and thrombin time in plasma samples from healthy dogs. Res Vet Sci 67:273–276. https://doi.org/10.1053/rvsc.1999.0321
Nikolac N (2014) Lipemia: causes, interference mechanisms, detection and management. Biochem Medica 24:57–67. https://doi.org/10.11613/BM.2014.008
Ohmori K, Nishikawa S, Oku K, Oida K, Amagai Y, Kajiwara N, Jung K, Matsuda A, Tanaka A, Matsuda H (2013) Circadian rhythms and the effect of glucocorticoids on expression of the clock gene period1 in canine peripheral blood mononuclear cells. Vet J 196:402–407. https://doi.org/10.1016/J.TVJL.2012.10.010
Radin MJ (2012) Laboratory evaluation of lipids. In: Thrall MA, Weiser G, Allison RW, Campbell TW (eds) Veterinary Hematology and Clinical Chemistry, 2nd edn. John Wiley & Sons, Ames, pp 480–496
Rizzi TE, Meinkoth JH, Clinkenbeard KD (2010) Normal hematology of the dog. In: Weiss DJ, Wardrop KJ (eds) Schalm’s Veterinary Hematology, 6th edn. Wiley-Blackwell, Philadelphia, pp 799–810
Schiettecatte J, Anckaert E, Smitz J (2012) Interferences in immunoassays. In: Chiu NHL (ed) Advances in Immunoassay Technology. InTech, Shanghai, pp 45–62
Silva Júnior JW, Borges FMO, Murgas LDS et al (2004) Digestibiliade de dietas com diferentes fontes de carboidratos e sua influência na glicemia e insulinemia em cães. Ciênc Agrotecnol Lavras 29:436–443
Silva NLT, Bonatto NCM, Oliveira PL, Vieira GC, Floriano BP, Barros LD, Bosculo MRM, Almeida BFM (2019) Post-prandial lipemia and glycemia in dogs fed with industrialized pet food. Comp Clin Path 28:253–258. https://doi.org/10.1007/s00580-018-2824-0
Simundic AM, Nikolac N, Vukasovic I, Vrkic N (2010) The prevalence of preanalytical errors in a Croatian ISO 15189 accredited laboratory. Clin Chem Lab Med 48:1009–1014. https://doi.org/10.1515/CCLM.2010.221
Watson AD, Church DB, Fairburn AJ (1981) Postprandial changes in plasma urea and creatinine concentrations in dogs. Am J Vet Res 42:1878–1880
Xenoulis PG, Steiner JM (2015) Canine hyperlipidaemia. J Small Anim Pract 56:595–605. https://doi.org/10.1111/jsap.12396
Zań RS, Roliński Z, Kowalski CJ, Bojarska-Junak A, Madany J (2013) Diurnal and seasonal changes in endogenous melatonin levels in the blood plasma in dogs. Pol J Vet Sci 16:759–761. https://doi.org/10.2478/pjvs-2013-0110
Acknowledgments
The authors are grateful to the São Paulo Research Foundation for the scholarship granted to the first author (FAPESP no. 2017/04175-0 and 2017/05105-6).
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This study was supported by the São Paulo Research Foundation (FAPESP no. 2017/04175-0 and 2017/05105-6).
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All applicable international, national, and institutional guidelines for the care and use of animals were followed. All practices were performed in accordance with the principles and guidelines of Ethics Committee on Animal Usage from the University Center of the Integrated Faculties of Ourinhos (protocol no. 023/2016). This article does not contain any studies with human participants performed by any of the authors
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Oliveira, P.L., Bonatto, N.C.M., Bosculo, M.R.M. et al. Effect of post-prandial lipemia on canine biochemical parameters. Comp Clin Pathol 29, 763–775 (2020). https://doi.org/10.1007/s00580-020-03130-y
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DOI: https://doi.org/10.1007/s00580-020-03130-y