Abstract
The escalating loss of biological diversity throughout the world has become a major concern for the conservation biologists. Like other threatened plant species, the natural populations of the orchids are also severely threatened. Dendrobium thyrsiflorum is one such representative of the family Orchidaceae whose natural populations are getting destroyed at an alarming rate and deserves special conservation attention. Both direct shoot organogenesis (DSO) and indirect shoot organogensesis (ISO) pathways were experimented and the highest regeneration frequency for DSO and ISO pathways were found to be 86.2 and 96 % respectively. The regenerated shoots were best rooted in half-strength MS medium supplemented with 1 mg/l indole butyric acid (IBA) and 0.5 mg/l phloroglucinol. The genetic stability of the acclimatized plants derived from ISO and DSO pathways was assessed using Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT) molecular markers. SCoT proved to be a superior marker over ISSR in detecting clonal variability. The phytochemical analysis of the micropropagated plants also revealed a comprehensively higher yield of various secondary metabolites with significantly higher antioxidant potentials in both ISO- and DSO-derived plants over the mother plant. However, the ISO-derived plants were more phytochemically enriched compared to the DSO-plants. The rapid multiplication rate, higher genetic stability and secondary metabolite production ensures the utility of this micropropagation method for D. thyrsiflorum in the ex situ conservation and commercial exploitation of other important orchid species.
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Acknowledgments
This research work was supported by the Centre for Advanced Studies (CAS) in Botany, University Grants Commission (UGC), India and the research Grant to PB in the form of Meritorious Student Fellowship (No. 42-47/Acad/Meritorious/Botany/2009-699) is gratefully acknowledged. PB also acknowledges Prof. Nirmalendu Saha, Mr. Bodhisatwa Banerjee and Mr. Debaprasad Koner, Department of Zoology, Northeastern Hill University, Shillong for the technical assistance provided during the course of this study.
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Bhattacharyya, P., Kumaria, S., Job, N. et al. Phyto-molecular profiling and assessment of antioxidant activity within micropropagated plants of Dendrobium thyrsiflorum: a threatened, medicinal orchid. Plant Cell Tiss Organ Cult 122, 535–550 (2015). https://doi.org/10.1007/s11240-015-0783-6
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DOI: https://doi.org/10.1007/s11240-015-0783-6