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Cytogenetic and Molecular Marker-Based Characterization of a Wheat-Psathyrostachys huashanica Keng 2Ns(2D) Substitution Line

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Abstract

Wheat-Psathyrostachys huashanica Keng substitution line 16-6, which possessed superior numbers of florets and kernels per spike, was developed from a cross between common wheat cv. 7182 and P. huashanica via embryo culture. This line was characterized based on cytological, genomic in situ hybridization (GISH), simple sequence repeat (SSR), fluorescent in situ hybridization (FISH), expressed sequence tag-sequence-tagged site (EST-STS), and morphological analyses. Line 16-6 contained 21 bivalents during meiosis, while GISH studies detected an alien chromosome pair substitution. Sixteen microsatellite markers for chromosome arms 2DS and 2DL of hexaploid wheat and FISH using the probe pAs1 indicated that chromosome 2D was missing. P. huashanica 2Ns(2D) chromosome introgression was confirmed using 780 EST-STS multiple-loci markers that covered all seven homoeologous groups and 14 EST-STS markers associated with homoeologous group 2 of wheat. After inoculation using mixed races of stripe rust (CYR31, CYR32, and SY11-14) in the adult stages, line 16-6 exhibited resistance, which was derived from the donor species. Compared with the control, the spike length, number of spikelets per spike, and kernel number per spike were significantly higher.

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Acknowledgments

Much appreciated financial support was provided by the Ministry of Agriculture “948” project of the People’s Republic of China (No. 2013-Z28), the Shaanxi Natural Science Foundation (No. 2012JM3001 and No. 2013JZ007), the Major Innovation Project for Science and Technology of Shaanxi Province (No. 2011KTZB02-01), the Basic Scientific Research Project in Central Universities (No. QN2011001), and the Tang Zhong-Ying Breeding Funding Project of the Northwest A&F University. The authors would like to thank Dr Duncan E. Jackson for useful advice and English language editing of the manuscript.

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  1. Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Wanli Du, Jixin Zhao, Jing Wang, Liangming Wang, Jun Wu, Qunhui Yang, Shuhui Liu & Xinhong Chen

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  1. Wanli Du
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  2. Jixin Zhao
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  7. Shuhui Liu
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  8. Xinhong Chen
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Correspondence to Xinhong Chen.

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Du, W., Zhao, J., Wang, J. et al. Cytogenetic and Molecular Marker-Based Characterization of a Wheat-Psathyrostachys huashanica Keng 2Ns(2D) Substitution Line. Plant Mol Biol Rep 33, 414–423 (2015). https://doi.org/10.1007/s11105-014-0761-x

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