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Molecular mapping and physical location of major gene conferring seedling resistance to Septoria tritici blotch in wheat

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Abstract

Septoria tritici blotch (STB) caused by Mycosphaerella graminicola (anamorph: Septoria tritici), is one of the most important foliar diseases of wheat. We assessed three doubled-haploid (DH) populations derived from Chara (STB-susceptible)/WW2449 (STB-resistant), Whistler (STB-susceptible)/WW1842 (STB-resistant) and Krichauff (STB susceptible)/WW2451 (STB-resistant) for resistance to a single-pycnidium isolate 79.2.1A of M. graminicola at the seedling stage. STB resistance in each of the three DH populations was conditioned by a single major gene designated as StbWW2449, StbWW1842 and StbWW2451. Linkage analyses and physical mapping indicated that the StbWW loci were located on the short arm of chromosome 1B (IBS). Four simple sequence repeat (SSR) markers linked with STB resistance: Xwmc230, Xbarc119b, Xksum045 and Xbarc008 were located to the distal bin of 1BS.sat1BS-4 (FL: 0.52–1.00) in the 1BS physical map. Xwmc230, Xbarc119b and Xksum045 markers, mapped within 7 cM from StbWW were validated for their linkage and predicted the STB resistance with over 94% accuracy in the 79 advanced breeding lines having WW2449 as one of the parents. The marker interval Xwmc230/Xksum045-Xbarc119b also explained up to 38% of the phenotypic variance at the adult plant stage in all three DH mapping populations. These results have proven that SSR markers are useful in monitoring STB resistance both at seedling and adult plant stages and hence are suitable for routine marker-assisted selection in the wheat breeding programs.

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Acknowledgments

We thank Drs Evans Lagudah (CSIRO Plant Industries, Australia and BS. Gill (Kansas State University, USA) for providing seeds of the wheat aneuploid lines. HR also thanks Dr KS. Gill (Washington State University, USA) for suggestions. This research work was supported by the NSW Agricultural Genomics Centre funded under the NSW Government’s BioFirst Initiative, Grains Research and Development Corporation, Value Added Wheat CRC and Enterprise Grains Australia. Technical assistance in STB evaluation tests conducted at the Wagga Wagga Agricultural Institute was provided by the late Mrs. Zdenka Tomes.

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Authors and Affiliations

  1. NSW Agricultural Genomics Centre and NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, 2650, Australia

    R. Raman & H. Raman

  2. NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Pine Gully Road, Wagga Wagga, NSW, 2650, Australia

    A. W. Milgate, M. Imtiaz, H. Raman, C. Lisle, N. Coombes & P. Martin

  3. Value Added Wheat CRC and NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Road, Menangle, NSW, 2570, Australia

    M.-K. Tan

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  1. R. Raman
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Correspondence to H. Raman.

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Raman, R., Milgate, A.W., Imtiaz, M. et al. Molecular mapping and physical location of major gene conferring seedling resistance to Septoria tritici blotch in wheat. Mol Breeding 24, 153–164 (2009). https://doi.org/10.1007/s11032-009-9280-0

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