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A Quninolylthiazole Derivatives as an ICT-Based Fluorescent Probe of Hg(II) and its Application in Ratiometric Imaging in Live HeLa Cells

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Abstract

As a structural analogue of pyridylthiazole, 2-(2-benzothiazoyl)-phenylethynylquinoline (QBT) was designed as a fluorescent probe for Hg(II) based on an intramolecular charge transfer (ICT) mechanism. The compound was synthesized in three steps starting from 6-bromo-2-methylquinoline, with moderate yield. Corresponding studies on the optical properties of QBT indicate that changes in the fluorescence ratio of QBT in response to Hg(II) could be quantified based on dual-emission changes. More specifically, the emission spectrum of QBT before and after interactions with Hg(II) exhibited a remarkable red shift of about 120 nm, which is rarely reported in ICT-based fluorescent sensors. Finally, QBT was applied in the two-channel imaging of Hg(II) in live HeLa cells.

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Acknowledgements

We thank the financial support from the National Natural Science Foundation of China (NSFC 21062023 and 21768004).

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Authors and Affiliations

  1. Research Centre of Chemical Biology, Yanbian University, Yanji, 133002, China

    Jian-Ying Bai, Yu-Zhong Xie, Lin-Nan Cao, Ling-Li Wang & Jing-Yi Jin

  2. National Demonstration Centre for Experimental Chemistry Education (Yanbian University), Department of Chemistry, Yanbian University, Yanji, 133002, China

    Jian-Ying Bai & Jing-Yi Jin

  3. College of Chemistry, Northeast Normal University, Changchun, 130024, China

    Yu-Zhong Xie

  4. Department of Nephrology, The Central Hospital of Jiamusi, Jiamusi, 154002, China

    Chang-Jiang Wang & Shu-Qing Fang

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  1. Jian-Ying Bai
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Correspondence to Jing-Yi Jin.

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Bai, JY., Xie, YZ., Wang, CJ. et al. A Quninolylthiazole Derivatives as an ICT-Based Fluorescent Probe of Hg(II) and its Application in Ratiometric Imaging in Live HeLa Cells. J Fluoresc 28, 795–800 (2018). https://doi.org/10.1007/s10895-018-2241-4

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  • DOI: https://doi.org/10.1007/s10895-018-2241-4

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