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A Dansyl-Rhodamine Ratiometric Fluorescent Probe for Hg2+ Based on FRET Mechanism

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Abstract

Based on resonance energy transfer (FRET) from dansyl to rhodamine 101, a new fluorescent probe (compound 1) containing rhodamine 101 and a dansyl unit was synthesized for detecting Hg2+ through ratiometric sensing in DMSO aqueous solutions. This probe shows a fast, reversible and selective response toward Hg2+ in a wide pH range. Hg2+ induced ring-opening reactions of the spirolactam rhodamine moiety of 1, leading to the formation of fluorescent derivatives that can serve as the FRET acceptors. Very large stokes shift (220 nm) was observed in this case. About 97-fold increase in fluorescence intensity ratio was observed upon its binding with Hg2+.

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Acknowledgments

The authors thank the NSFC (project No. 21102037) and the Education Department of Henan Province (No. 2010GGJS-048) for the financial support.

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Authors and Affiliations

  1. College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Puhui Xie, Sen Yang & Guoyu Yang

  2. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Fengqi Guo, Lingyu Wang & Denghui Yao

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  1. Puhui Xie
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  2. Fengqi Guo
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  3. Lingyu Wang
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  4. Sen Yang
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Correspondence to Puhui Xie or Fengqi Guo.

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Xie, P., Guo, F., Wang, L. et al. A Dansyl-Rhodamine Ratiometric Fluorescent Probe for Hg2+ Based on FRET Mechanism. J Fluoresc 25, 319–325 (2015). https://doi.org/10.1007/s10895-015-1511-7

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