Abstract
Heptacodium miconioides Rehd. is an endangered species endemic to China and has suffered rapid decrease of distribution range and population size. This species has been disappeared in central China where the modal specimen was collected. We analyzed the genetic variation of the remaining populations to reveal whether the genetic diversity also suffered decrease and to provide some suggestions for conservation. All the nine known remaining populations were sampled. Genetic variation was analyzed based on RAPD markers and two fragments of cpDNA sequence, intergenic spacers of petG-trnP and trnS-trnG. No variation was observed in the two fragments of cpDNA sequence. However, the species exhibited high level of RAPD variation compared to other threatened or rare plants. Measures of genetic diversity within populations were strongly related to the log of estimated population size, indicating that large populations usually have more genetic diversity than that of small ones. About 25% of the variation was partitioned among populations. Significant relationship was observed between differentiation and geographical distance, indicating a pattern of isolation-by-distance. Given for few populations remaining, all the populations should be protected and urgent efforts be paid on the small populations to avoid their local extinction.
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Allnutt TR, Newton AC, Premoli A, Lara A (2003) Genetic variation in the threatened South American conifer Pilgerodendron uviferum (Cupressaceae), detected using RAPD markers. Biol Conserv 114:245–253
Alvarez-Buylla ER, Garcia-Barrios R, Lara-Moreno C, Martinez-Ramos M (1996) Demographic and genetic models in conservation biology: applications and perspectives for tropical rain forest tree species. Annu Rev Ecol Syst 27:387–421
Bell CD, Edwards EJ, Kim ST, Donoghue MJ (2001) Dipsacales phylogeny based on chloroplast DNA sequences. Harvard Pap Bat 6:481–499
Bian CM, Jin ZX, Li JM (2002) A study on the reproductive biology of Heptacodium miconioides. Acta Bot Yunnanica 24:613–618
Buchert GP, Rajora OP, Hood JV, Dancik BP, (1997) Effects of harvesting on genetic diversity in old-growth eastern white pine in Ontario, Canada. Conserv Biol 11:747–758
Cardoso MA, Provan J, Powell W, Ferreira PCG, de Oliveira DE (1998) High genetic differentiation among remnant populations of the endangered Caesalpinia echinata Lam (Leguminosae–Caesalpinioideae). Mol Ecol 7:601–608
Chen XY (2000) Effects of habitat fragmentation on genetic structure of plant populations and implications for the biodiversity conservation. Acta Ecol Sin 20:884–892
Chen XY, Lu HP, Shen L, Li YY (2002) Identifying populations for priority conservation of important species. Biodivers Sci 10:332–338
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Ellstrand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol Syst 24:217–242
Excoffier L (1993) AMOVA: analysis of molecular variance (ver 1.55). Genetics and Biometry Laboratory, University of Geneva
Fan XX, Shen L, Zhang X, Chen XY, Fu CX (2004) Assessing genetic diversity of Ginkgo biloba L (Ginkgoaceae) populations from China by RAPD markers. Biochem Genet 42:269–278
Frankham R (1996) Relationship of genetic variation to population size in wildlife. Conserv Biol 10:1500–1508
Franklin IR (1980) Evolutionary change in small populations. In: Soule ME, Wilcox B (eds) Conservation biology: an evolutionary-ecological perspective. Sinauer Associates, Sunerland, pp 135–149
Fu LG, Jin JM (1992) Red list of endangered plants in China. Science Press, Beijing
Hamilton MB (1999) Four primer pairs for the amplification of chloroplast intergenic regions with intraspecific variation. Mol Ecol 8:521–523
Huang SSF, Hwang SY, Lin TP (2002) Spatial pattern of chloroplast DNA variation of Cyclobalanopsis glauca in Taiwan and East Asia. Mol Ecol 11:2349–2358
Jin Z (1996) A preliminary study on phytocoenosis features of Heptacodium miconioides community in Tiantai Mountains of Zhejiang Province. Guihaia 16:25–34
Johnson NC (1995) Biodiversity in the balance: approaches to setting geographic conservation priorities. Biodiversity Support Program, Washington, DC
Kark S, Alkon PU, Safriel UN, Randi E (1999) Conservation priorities for Chukar Partridge in Israel based on genetic diversity across an ecological gradient. Conserv Biol 13:542–552
Kwon JA, Morden CW (2002) Population genetic structure of two rare tree species (Colubrina oppositifolia and Alphitonia ponderosa, Rhamnaceae) from Hawaiian dry and mesic forests using random amplified polymorphic DNA markers. Mol Ecol 11:991–1001
Lande R (1995) Mutation and conservation. Conserv Biol 9:782–791
Lewontin RC (1972) The apportionment of human diversity. Evo1 Biol 6:381–398
Li M, Gu YJ, Zhang X, Chen XY (2004) Population structure of Heptacodium miconioides, an endangered species, in Dapan Mountain of Zhejiang Province. J East China Norm Uni (Natl Sci) (4):117–121
Li YY, Chen XY, Zhang X, Wu TY, Lu HP et al. (2005) Genetic differences between wild and artificial populations of Metasequoia glyptostroboides Hu et Cheng (Taxodiaceae): implications for species recovery. Conserv Biol 19:224–231
Lynch M, Lande R (1998) The critical size for a genetically secure population. Anim Conserv 1:70–72
Lynch M., Milligan BG (1994) Analysis of population genetic structure with RAPD markers. Mol Ecol 3:91–99
Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220
Martin APMS, Adamec L, Suda J, Mes THM, Storchova H (2003) Genetic variation within the endangered species Aldrovanda vesiculosa (Droseraceae) as revealed by RAPD analysis. Aquat Bot 75:159–172
Miller MP (1997) Tools for population genetic analyses (TFPGA) vl.3: a windows program for the analysis of allozyme and molecular genetic data. Department of Biological Sciences, Northern Arizona University, Flagstaff
Miller MP (1998) AMOVA-PREP, a program for the preparation of AMOVA input files from dominant marker raw data, release 1.01. Department of Biological Sciences, Northern Arizona University, Flagstaff
Mogensen HL (1996) The hows and whys of cytoplasmic inheritance in seed plants. Am J Bot 83:383–404
Morden CW, Loeffler W (1999) Fragmentation and genetic differentiation among subpopulations of the endangered Hawaiian mint Haplostachys haplostachya (Lamiaceae). Mol Ecol 8:617–625
Mueller-Scharer H, Fischer M (2001) Genetic structure of the annual weed Senecio vulgaris in relation to habitat type and population size. Mol Ecol 10:17–28
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Newton AC, Allnutt TR, Dvorak WS, Del Castillo RF, Ennos RA (2002) Patterns of genetic variation in Pinus chiapensis, a threatened Mexican pine, detected by RAPD and mitochondrial DNA RFLP markers. Heredity 89:191–198
Newton AC, Allnutt TR, Gillies ACM, Lowe AJ, Ennos RA (1999) Molecular phylogeography, intraspecific variation and the conservation of tree species. Trends Ecol Evol 14:140–145
Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155
O’Brien S (1994) A role for molecular genetics in biological conservation. Proc Natl Acad Soc USA 91:5748–5755
Petit RJ, Mousadik AE, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv Biol 12:844–855
Prathepha P, Baimai V (1999) Genetic differentiation in Thai populations of the rare species Afgekia sericea Craib (Leguminosae) revealed by RAPD–PCR assays. Genetica 105:193–202
Pyck N, Smets E (2000) A search for the phylogenetic position of the seven-son-flower (Heptacodium, Dipsacales): combining molecular and morphological evidence. Plant Syst Evol 225:185–199
Shi YF, Cui ZJ, Li JJ (1989) Quaternary glacials and environmental issues of Eastern China. Science Press, Beijing
Sork VL, Nason J, Campbell DR, Fernandez JF (1999) Landscape approaches to historical and contemporary gene flow in plants. Trends Ecol Evol 14:219–224
Sun M, Wong KC (2001) Genetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers. Am J Bot 88:2180–2188
Tang YC, Li LQ (1994) The phytogeography of Caprifoliaceae s str with its implications for understanding eastern Asiatic flora. Acta Phytotaxon Sin 32:197–218
Yeh FC, Yang RC, Boyle T (1999) Popgene version 1.31: microsoft Window-based freeware for population genetic analysis. Department of Renewable Resources, University of Alberta, Edmonton, AB Canada
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Lu, HP., Cai, YW., Chen, XY. et al. High RAPD but no cpDNA sequence variation in the endemic and endangered plant, Heptacodium miconioides Rehd. (Caprifoliaceae). Genetica 128, 409–417 (2006). https://doi.org/10.1007/s10709-006-7542-x
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DOI: https://doi.org/10.1007/s10709-006-7542-x