Skip to main content
SpringerLink
Log in

Cyanobactericidal effect of Rhodococcus sp. isolated from eutrophic lake on Microcystis sp

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A bacterium, which was observed in all cultivations of Microcystis sp., was isolated and designated as Rhodococcus sp. KWR2. The growth of bloom-forming cyanobacteria, including four strains of Microcystis aeruginosa and Anabaena variabilis, was suppressed by up to 75–88% by 2% (v/v) culture broth of KWR2 after 5 days. But KWR2 did not inhibit eukaryotic algae, Chlorella vulgaris and Scenedesmus sp. An extracellular algicidal substance produced by KWR2 showed a cyanobactericidal activity of 94% and was water-soluble with a molecular weight of lower than 8 kDa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ahn CY, Joung SH, Jeon JW, Kim HS, Yoon BD, Oh HM (2003) Selective control of cyanobacteria by surfactin-containing culture broth of Bacillus subtilis C1. Biotechnol Lett 25:1137–1142

    Article  PubMed  CAS  Google Scholar 

  • Allen MM (1968) Simple conditions for the growth of unicellular blue-green algae on plates. J Phycol 4:1–4

    Article  CAS  Google Scholar 

  • Daft MJ, McCord SB, Stewart WDP (1975) Ecological studies on algal-lysing bacteria in fresh waters. Freshw Biol 5:577–596

    Article  Google Scholar 

  • Doi RH (1983) Chromosomal DNA techniques. In: Rodriguez RL (ed) Recombinant DNA techniques. Addison-Wesley, MA, pp 161–163

    Google Scholar 

  • Doucette GJ, Kodama M, Franca S, Gallacher S (1998) Bacterial interactions with harmful algal bloom species: bloom ecology toxigenesis and cytology. In: Anderson DM (ed) Physiological ecology of harmful algal blooms. Springer-Verlag, Berlin, pp 619–647

    Google Scholar 

  • Fukami K, Yuzawa A, Nishijima T, Hata Y (1992) Isolation and properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi 58:1073–1077

    Google Scholar 

  • Gotham IJ, Rhee GY (1982) Effects of nitrate and phosphate limitation on cyclostat growth of two freshwater diatoms. J Gen Microbiol 128:199–205

    CAS  Google Scholar 

  • Hua XH, Li JH, Li JJ, Li HZ, Cui Y (2009) Selective inhibition of the cyanobacterium, Microcystis, by a Streptomyces sp. Biotechnol Lett 31:1531–1535

    Article  PubMed  CAS  Google Scholar 

  • Jung SW, Kim BH, Katano T, Kong DS, Han MS (2008) Pseudomonas fluorescens HYK0210-SK09 offers species specific biological control of winter algal blooms caused by freshwater diatom Stephanodiscus hantzschii. J Appl Microbiol 105:186–195

    Article  PubMed  CAS  Google Scholar 

  • Lee SO, Kato J, Takiguchi N, Kuroda A, Ikeda T, Mitsutani A, Ohtake H (2000) Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl Environ Microbiol 66:4334–4339

    Article  PubMed  CAS  Google Scholar 

  • Mayali X, Azam F (2004) Algicidal bacteria in the sea and their impact on algal blooms. J Eukaryot Microbiol 51:139–144

    Article  PubMed  Google Scholar 

  • McGuire MJ, Jones RM, Means EG, Izaguirre G, Preston AE (1984) Controlling attached blue-green algae with copper sulfate. J Am Water Works Assoc 76:60–65

    CAS  Google Scholar 

  • Muyzer G, de Waal E, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700

    PubMed  CAS  Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Genetic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Takamura Y, Takashi Y, Akiko K, Naomi K, Tetsuya T, Shiro N, Osami Y (2004) Growth inhibition of Microcystis cyanobacteria by L-lysine and disappearance of natural Microcystis blooms with spraying. Microbes Environ 19:31–39

    Article  Google Scholar 

  • Wood LW (1985) Chloroform-methanol extraction of chlorophyll-a. Can J Fish Aquat Sci 42:38–43

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program, the MEST/NRF to the Environment Biotechnology National Core Research Center (grant #: 20090091489), and to the Center for Aquatic Ecosystem Restoration, Eco-STAR project from the Ministry of Environment, Republic of Korea.

Author information

Authors and Affiliations

  1. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea

    Young-Ki Lee, Chi-Yong Ahn, Hee-Sik Kim & Hee-Mock Oh

Authors
  1. Young-Ki Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chi-Yong Ahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee-Sik Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hee-Mock Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hee-Mock Oh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, YK., Ahn, CY., Kim, HS. et al. Cyanobactericidal effect of Rhodococcus sp. isolated from eutrophic lake on Microcystis sp. Biotechnol Lett 32, 1673–1678 (2010). https://doi.org/10.1007/s10529-010-0350-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-010-0350-5

Keywords

Search

Navigation