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Effect of supplementary manganese on growth performance, tissue manganese content, antioxidant activities, and cytosolic manganese superoxide dismutase gene mRNA expression in juvenile Chinese mitten crabs (Eriocheir sinensis)

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Abstract

A 50-day feeding trial was conducted to determine the dietary manganese (Mn) requirement of juvenile Chinese mitten crabs Eriocheir sinensis. Manganese sulfate monohydrate (MnSO4·H2O) was added to a semi-purified basal diet to prepare six experimental diets with graded levels of Mn (0, 10, 20, 40, 80, and 160 mg/kg) providing the actual dietary Mn value of 2.12, 12.26, 22.45, 33.78, 44.05, and 66.18 mg/kg. Results showed that Mn supplementation significantly (P < 0.05) increased the weight growth rate (WGR), feed efficiency (FE), and activities of both hepatic Mn superoxide dismutase (Mn-SOD) and total SOD (T-SOD). Survival rate was not affected by supplementary Mn (P > 0.05). Mn concentration in the whole body and hepatopancreas significantly increased with increasing levels of dietary Mn supplementation. However, crabs fed an additional 2.12 mg/kg of Mn had a significantly (P < 0.05) higher Mn retention and proportion of hepatic Mn to whole-body Mn than other groups. As biomarkers of oxidative stress, malondialdehyde content in the serum was significantly higher in crabs fed the basal diet (P < 0.05). The mRNA expression of the cytosolic Mn-SOD gene in the hepatopancreas was upregulated with increasing dietary Mn levels up to 22.45 mg/kg and then plateaued. Using the broken-line model based on WGR, the dietary Mn requirement of the juvenile Chinese mitten crab was estimated to be 30.34 mg/kg.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (grant numbers 31560726, 31260642, 31402305), the Major Project of Science and Technology of Jiangxi Province of China (2016ACF60018), the Natural Science Foundation of Jiangxi Province of China (grant number 20132BAB214015), and the Educational Commission of Sichuan Province of China (grant number 14ZA0249).

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Authors and Affiliations

  1. School of Life Sciences, Nanchang University, Nanchang, Jiangxi, 330013, People’s Republic of China

    Daxian Zhao, Zhenfang Mao, Qichen Lin & Yijiang Hong

  2. Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, College of Life Science, Neijiang Normal University, Neijiang, 641000, People’s Republic of China

    Chuanjie Qin

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  1. Daxian Zhao
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  2. Zhenfang Mao
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Correspondence to Yijiang Hong.

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Zhao, D., Mao, Z., Lin, Q. et al. Effect of supplementary manganese on growth performance, tissue manganese content, antioxidant activities, and cytosolic manganese superoxide dismutase gene mRNA expression in juvenile Chinese mitten crabs (Eriocheir sinensis). Aquacult Int 25, 893–903 (2017). https://doi.org/10.1007/s10499-016-0086-x

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