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Studying the Influence of Copper on the Growth Behavior, Antioxidative Status, and Histology of the Intestine and Liver of Striped Catfish (Pangasianodon hypophthalmus)

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Abstract

Microelements are well recognized as an essential approach in the field of aquaculture nutrition. Thus, this study aimed to evaluate copper (Cu) inclusion (0, 0.5, 1, and 2 mg/kg) on Striped catfish performances. Fish fed the Cu-incorporated diets for 60 days, then their growth behavior, antioxidative capacity, and intestinal and liver histological features were evaluated. The results showed a marked enhancement in Striped catfish's growth behavior fed 1–2 mg/kg of Cu, as shown by the final weight, weight gain, and specific growth rate. The feed and protein efficiency ratios were significantly affected by Cu in a dose-dependent manner. The highest level of Cu was accumulated in the whole body, muscle, liver, and gills of fish fed 2 mg/kg of Cu. The carcass composition of Striped catfish showed higher protein content in groups received 0.5, 1, and 2 mg/kg Cu in a linear and quadratic manner (p=0.001). The ash content was quadratically increased in Striped catfish fed 2 mg/kg Cu (p=0.001). However, no marked effects were observed on the moisture and lipid contents and the somatic indices (p>0.05). The incorporation of Cu showed meaningfully increased superoxide dismutase, catalase, and glutathione peroxidase but decreased malondialdehyde level in Striped catfish. The villous height exhibited visible growth and branching with increased doses of Cu without a significant increase in the goblet cells. No abnormal features were observed in the liver and hepatocytes of fish treated with Cu. It can be concluded that Cu is required at 1–2 mg/kg for better performances of Striped catfish.

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References

  1. Davis DA, Gatlin DM (1996) Dietary mineral requirements of fish and marine crustaceans. Rev Fish Sci 4:75–99

    Article  Google Scholar 

  2. Dawood MAO (2021) Nutritional immunity of fish intestines: important insights for sustainable aquaculture. Rev Aquac 13:642–663

    Article  Google Scholar 

  3. Moyson S, Liew HJ, Fazio A, Van Dooren N, Delcroix A, Faggio C et al (2016) Kidney activity increases in copper exposed goldfish (Carassius auratus auratus). Comp Biochem Physiol C Toxicol Pharmacol 190:32–37

    Article  CAS  Google Scholar 

  4. Shi B, Yuan Y, Jin M, Betancor MB, Tocher DR, Jiao L, Song D, Zhou Q (2021) Transcriptomic and physiological analyses of hepatopancreas reveal the key metabolic changes in response to dietary copper level in Pacific white shrimp Litopenaeus vannamei. Aquaculture. 532:736060

    Article  CAS  Google Scholar 

  5. Watanabe T, Kiron V, Satoh S (1997) Trace minerals in fish nutrition. Aquaculture. 151:185–207

    Article  CAS  Google Scholar 

  6. Lall SP (2002) The minerals. In: Halver JE, Hardy RW (eds) Fish nutrition. Academic Press, New York, pp 259–308

    Google Scholar 

  7. Abdel-Hameid N-AH, Zehra S, Khan MA (2017) Dietary copper requirement of fingerling Channa punctatus (Bloch) based on growth, feed conversion, blood parameters and whole body copper concentration. Aquac Res 48:2787–2797

    Article  CAS  Google Scholar 

  8. Shiau SY, Ning YC (2003) Estimation of dietary copper requirements of juvenile tilapia, Oreochromis niloticus × O. aureus. Anim Sci 77:287–292

    Article  CAS  Google Scholar 

  9. Chen K, Yamamoto FY, Gatlin DM III (2020) Effects of inorganic and organic dietary copper supplementation on growth performance and tissue composition for juvenile red drum (Sciaenops ocellatus L.). Aquac Nutr 26:820–827

  10. Lin Y-H, Shih C-C, Kent M, Shiau S-Y (2010) Dietary copper requirement reevaluation for juvenile grouper, Epinephelus malabaricus, with an organic copper source. Aquaculture. 310:173–177

    Article  CAS  Google Scholar 

  11. Cao J, Miao X, Xu W, Li J, Zhang W, Mai K (2014) Dietary copper requirements of juvenile large yellow croaker Larimichthys croceus. Aquaculture. 432:346–350

    Article  CAS  Google Scholar 

  12. Tan XY, Luo Z, Liu X, Xie CX (2011) Dietary copper requirement of juvenile yellow catfish Pelteobagrus fulvidraco. Aquac Nutr 17:170–176

    Article  CAS  Google Scholar 

  13. Wang H, Li E, Zhu H, Du Z, Qin J, Chen L (2016) Dietary copper requirement of juvenile Russian sturgeon Acipenser gueldenstaedtii. Aquaculture. 454:118–124

    Article  CAS  Google Scholar 

  14. Liang H, Ji K, Ge X, Mi H, Xi B, Ren M (2020) Effects of dietary copper on growth, antioxidant capacity and immune responses of juvenile blunt snout bream (Megalobrama amblycephala) as evidenced by pathological examination. Aquac Rep 17:100296

    Article  Google Scholar 

  15. Xu Z, Wang Y, Gul Y, Li Q, Song J, Hu M (2020) Effects of copper supplement on the immune function and blood-chemistry in adult Chinese horseshoe crab Tachypleus tridentatus. Aquaculture. 515:734576

    Article  CAS  Google Scholar 

  16. NRC (2011) National research council- nutrient requirements of fish and shrimp. National academies press

  17. Lorentzen, Maage, Julshamn (1998) Supplementing copper to a fish meal based diet fed to Atlantic salmon parr affects liver copper and selenium concentrations. Aquac Nutr 4:67–72

    Article  CAS  Google Scholar 

  18. Bharadwaj AS, Patnaik S, Browdy CL, Lawrence AL (2014) Comparative evaluation of an inorganic and a commercial chelated copper source in Pacific white shrimp Litopenaeus vannamei (Boone) fed diets containing phytic acid. Aquaculture. 422-423:63–68

    Article  CAS  Google Scholar 

  19. Bonham M, O'Connor JM, Hannigan BM, Strain J (2002) The immune system as a physiological indicator of marginal copper status? Br J Nutr 87:393–403

    Article  CAS  Google Scholar 

  20. Jayant M, Muralidhar AP, Sahu NP, Jain KK, Pal AK, Srivastava PP (2018) Protein requirement of juvenile striped catfish, Pangasianodon hypophthalmus. Aquac Int 26:375–389

    Article  CAS  Google Scholar 

  21. Allam BW, Khalil HS, Mansour AT, Srour TM, Omar EA, Nour AAM (2020) Impact of substitution of fish meal by high protein distillers dried grains on growth performance, plasma protein and economic benefit of striped catfish (Pangasianodon hypophthalmus). Aquaculture. 517:734792

    Article  CAS  Google Scholar 

  22. AOAC (2012) Official methods of analysis of AOAC international, 19th edn. AOAC International, Gaithersburg www.eoma.aoac.org

    Google Scholar 

  23. El Basuini MF, El-Hais AM, Dawood MA, Abou-Zeid AE-S, El-Damrawy SZ, Khalafalla MME-S et al (2016) Effect of different levels of dietary copper nanoparticles and copper sulfate on growth performance, blood biochemical profiles, antioxidant status and immune response of red sea bream (Pagrus major). Aquaculture. 455:32–40

    Article  Google Scholar 

  24. Ajani E, Akpoilih B (2012) Growth responses and ionic regulation in common carp (Cyprinus carpio) after chronic dietary copper exposure and recovery. J Environ Anal Toxicol 2:2161–0525.1000143

    Google Scholar 

  25. Bancroft J, Layton C (2013) Theory & practice of histological techniques, 7th edn. Churchill Livingstone of Elsevier, Philadelphia, pp 172–214

    Google Scholar 

  26. Gewaily MS, Shukry M, Abdel-Kader MF, Alkafafy M, Farrag FA, Moustafa EM et al (2021) Dietary Lactobacillus plantarum relieves Nile tilapia (Oreochromis niloticus) juvenile from oxidative stress, immunosuppression and inflammation induced by deltamethrin and Aeromonas hydrophila. Front Mar Sci 8:203

    Google Scholar 

  27. Truong Thy HT, Tri NN, Quy OM, Fotedar R, Kannika K, Unajak S, Areechon N (2017) Effects of the dietary supplementation of mixed probiotic spores of Bacillus amyloliquefaciens 54A, and Bacillus pumilus 47B on growth, innate immunity and stress responses of striped catfish (Pangasianodon hypophthalmus). Fish Shellfish Immunol 60:391–399

    Article  CAS  Google Scholar 

  28. Muralisankar T, Bhavan PS, Srinivasan V, Radhakrishnan S, Seenivasan C, Manickam N (2015) Effects of dietary copper on the growth physiology and biochemistry of the freshwater prawn Macrobrachium rosenbergii post larvae. Int J Pure Appl Biosci 3:509–518

    Google Scholar 

  29. Neves RCF, Moraes PM, Ferrari JEM, Lima PM, Santos FA, de Castro GR, Barros MM, Padilha PM (2011) Levels of copper in Nile tilapia from Brazil. Food Addit Contam Part B Surveill 4:238–243

    Article  CAS  Google Scholar 

  30. Mohseni M, Pourkazemi M, Bai SC (2014) Effects of dietary inorganic copper on growth performance and immune responses of juvenile beluga, Huso huso. Aquac Nutr 20:547–556

    Article  CAS  Google Scholar 

  31. Dawood MAO, El Basuini MF, Zaineldin AI, Yilmaz S, Hasan MT, Ahmadifar E et al (2021) Antiparasitic and antibacterial functionality of essential oils: an alternative approach for sustainable aquaculture. Pathogens. 10:185

    Article  CAS  Google Scholar 

  32. Magouz FI, Mahmoud SA, El-Morsy RA, Paray BA, Soliman AA, Zaineldin AI et al (2021) Dietary menthol essential oil enhanced the growth performance, digestive enzyme activity, immune-related genes, and resistance against acute ammonia exposure in Nile tilapia (Oreochromis niloticus). Aquaculture. 530:735944

    Article  CAS  Google Scholar 

  33. Castro C, Peréz-Jiménez A, Coutinho F, Corraze G, Panserat S, Peres H, Teles AO, Enes P (2018) Nutritional history does not modulate hepatic oxidative status of European sea bass (Dicentrarchus labrax) submitted to handling stress. Fish Physiol Biochem 44:911–918

    Article  CAS  Google Scholar 

  34. Paray BA, El-Basuini MF, Alagawany M, Albeshr MF, Farah MA, Dawood MAO (2021) Yucca schidigera usage for healthy aquatic animals: potential roles for sustainability. Animals. 11:93

    Article  Google Scholar 

  35. Shao X-p, Liu W-b, Xu W-n, Lu K-l, Xia W, Jiang Y-y (2010) Effects of dietary copper sources and levels on performance, copper status, plasma antioxidant activities and relative copper bioavailability in Carassius auratus gibelio. Aquaculture. 308:60–65

    Article  CAS  Google Scholar 

  36. Kovacik A, Tvrda E, Miskeje M, Arvay J, Tomka M, Zbynovska K, Andreji J, Hleba L, Kovacikova E, Fik M, Cupka P, Nahacky J, Massanyi P (2019) Trace metals in the freshwater fish Cyprinus carpio: effect to serum biochemistry and oxidative status markers. Biol Trace Elem Res 188:494–507

    Article  CAS  Google Scholar 

  37. Tang L, Huang K, Xie J, Yu D, Sun L, Huang Q, Bi Y (2017) Dietary copper affects antioxidant and immune activity in hybrid tilapia (Oreochromis niloticus × Oreochromis aureus). Aquac Nutr 23:1003–1015

    Article  CAS  Google Scholar 

  38. Pierri BS, Silva AD, Cadorin DI, Ferreira TH, Mouriño JLP, Filer K et al (2021) Different levels of organic trace minerals in diets for Nile tilapia juveniles alter gut characteristics and body composition, but not growth. Aquac Nutr 27:176–186

    Article  CAS  Google Scholar 

  39. Ratn A, Prasad R, Awasthi Y, Kumar M, Misra A, Trivedi SP (2018) Zn2+ induced molecular responses associated with oxidative stress, DNA damage and histopathological lesions in liver and kidney of the fish, Channa punctatus (Bloch, 1793). Ecotoxicol Environ Saf 151:10–20

    Article  CAS  Google Scholar 

  40. Aliko V, Hajdaraj G, Caci A, Faggio C (2015) Copper induced lysosomal membrane destabilisation in haemolymph cells of Mediterranean green crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania). Braz Arch Biol Technol 58:750–756

    Article  CAS  Google Scholar 

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Funding

The current work was funded by Taif University Researchers (Supporting Project number TURSP-2020/57), Taif University, P.O. Box 11099, Taif 21944, Taif, Saudi Arabia.

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Authors and Affiliations

  1. Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Mohamed E. El-Sharawy, Ahmed A. Mahmoud, Mohamed A. Mahmoud & Mahmoud A. O. Dawood

  2. Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Sakha Aquaculture Research Unit, Kafrelsheikh, Egypt

    Ali A. Soliman

  3. Fish Nutrition Laboratory, Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt

    Asem A. Amer

  4. Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia

    Mohamed Alkafafy

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  1. Mohamed E. El-Sharawy
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  2. Ahmed A. Mahmoud
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  7. Mahmoud A. O. Dawood
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Correspondence to Mahmoud A. O. Dawood.

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Animal Welfare Statement

The experimental procedure was approved by the ethics review board of the Institutional Animal Care and Use Committee in Kafrelsheikh University (Kafrelsheikh, Egypt).

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El-Sharawy, M.E., Mahmoud, A.A., Soliman, A.A. et al. Studying the Influence of Copper on the Growth Behavior, Antioxidative Status, and Histology of the Intestine and Liver of Striped Catfish (Pangasianodon hypophthalmus). Biol Trace Elem Res 200, 1331–1338 (2022). https://doi.org/10.1007/s12011-021-02717-y

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