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Analysis of cuproptosis-related lncRNA signature for predicting prognosis and tumor immune microenvironment in pancreatic cancer

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Abstract

Pancreatic cancer (PC) is a highly malignant digestive tract tumor, with a dismal 5-year survival rate. Recently, cuproptosis was found to be copper-dependent cell death. This work aims to establish a cuproptosis-related lncRNA signature which could predict the prognosis of PC patients and help clinical decision-making. Firstly, cuproptosis-related lncRNAs were identified in the TCGA-PAAD database. Next, a cuproptosis-related lncRNA signature based on five lncRNAs was established. Besides, the ICGC cohort and our samples from 30 PC patients served as external validation groups to verify the predictive power of the risk signature. Then, the expression of CASC8 was verified in PC samples, scRNA-seq dataset CRA001160, and PC cell lines. The correlation between CASC8 and cuproptosis-related genes was validated by Real-Time PCR. Additionally, the roles of CASC8 in PC progression and immune microenvironment characterization were explored by loss-of-function assay. As showed in the results, the prognosis of patients with higher risk scores was prominently worse than that with lower risk scores. Real-Time PCR and single cell analysis suggested that CASC8 was highly expressed in pancreatic cancer and related to cuproptosis. Additionally, gene inhibition of CASC8 impacted the proliferation, apoptosis and migration of PC cells. Furthermore, CASC8 was demonstrated to impact the expression of CD274 and several chemokines, and serve as a key indicator in tumor immune microenvironment characterization. In conclusion, the cuproptosis-related lncRNA signature could provide valuable indications for the prognosis of PC patients, and CASC8 was a candidate biomarker for not only predicting the progression of PC patients but also their antitumor immune responses.

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Data availability

The data used in this study are all available from the TCGA database (https://portal.gdc.cancer.gov/), Genome Sequence Archive (accession number: CRA001160) at https://bigd.big.ac.cn/bioproject/browse/PRJCA001063, and ICGC database (https://dcc.icgc.org/).

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The authors declare no acknowledgment.

Funding

This work was supported by the National Natural Science Foundation of China (Grant Number 81802317 to Min-Wei Yang; 82003166 to Ding M; 81902377 to De-Jun Liu.)

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  1. Hong-Fei Yao, Da-Peng Xu, Jia-Hao Zheng and Yu Xu have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Oncogenes and Related Genes, Department of Biliary-Pancreatic Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, People’s Republic of China

    Hong-Fei Yao, Da-Peng Xu, Jia-Hao Zheng, Yu Xu, Qin-Yuan Jia, Yu-Heng Zhu, Jian Yang, Rui-Zhe He, Ding Ma, Min-Wei Yang, Xue-Liang Fu, De-Jun Liu, Yan-Miao Huo, Jian-Yu Yang & Jun-Feng Zhang

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Contributions

Conceptualization, H-FY, D-PX; methodology, H-FY, D-PX, J-HZ, and YX; resources, H-FY, D-PX, J-HZ, and YX; writing—original draft preparation, H-FY, Q-YJ, Y-HZ, JY, R-ZH, X-LF; writing—review and editing, Y-MH, J-YY, J-FZ, Funding acquisition, M-WY, DM, D-JL; Supervision, Y-MH, J-YY, J-FZ. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Yan-Miao Huo, Jian-Yu Yang or Jun-Feng Zhang.

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The study was approved under the number RA-2019-116 assigned by the Research Ethics Committee of Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University.

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Yao, HF., Xu, DP., Zheng, JH. et al. Analysis of cuproptosis-related lncRNA signature for predicting prognosis and tumor immune microenvironment in pancreatic cancer. Apoptosis 28, 1090–1112 (2023). https://doi.org/10.1007/s10495-023-01843-3

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