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Alexandriicola marinus gen. nov., sp. nov., a new member of the family Rhodobacteraceae isolated from marine phycosphere

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Abstract

Two yellow-pigmented bacterial strains, LZ-14 T and ABI-LZ29, were isolated from the cultivable phycosphere microbiota of the highly toxic marine dinoflagellate Alexandrium catenella LZT09 and demonstrated obvious microalgae growth-promoting potentials toward the algal host. To elucidate the taxonomic status of the two bioactive bacterial strains, they were subjected to a polyphasic taxonomic characterization. Both strains were found to be Gram-negative, aerobic, rod-shaped and motile; to contain Q-10 as the predominant ubiquinone; summed feature 8, C16:0, C18:1 ω7c 11-methyl and summed feature 3 as the major fatty acids; and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids as the predominant polar lipids. Based on the phylogenetic analysis, phylogenomic inferences and phenotypic characteristics, the strains could be clearly distinguished from phylogenetically closely related species and formed a distinct monophyletic lineage in the family Rhodobacteraceae. The size of the draft genome of strain LZ-14 T is 4.615 Mb, with a DNA G + C content of 63.3 mol%. It contains ten predicted secondary metabolite biosynthetic gene clusters and core genes for bacterial exopolysaccharide biosynthesis. Therefore, strain LZ-14 T (= CCTCC AB 2017230 T = KCTC 62342 T) represents a novel species of a new genus, for which the name Alexandriicola marinus gen. nov., sp. nov., is proposed.

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Abbreviations

AAI:

Average amino acid identity

ABI:

Algae-bacteria interactions

ANI:

Average nucleotide identity

dDDH:

Digital DNA-DNA hybridization

GL:

Glycolipids

MA:

Marine agar

MB:

Marine broth

MEGA:

Molecular evolutionary genetics analysis

ML:

Maximum likelihood

MP:

Maximum parsimony

NJ:

Neighbour joining

POCP:

Percentage of conserved proteins

TLC:

Thin-layer chromatography

UBCG:

Up-to-date bacterial core gene

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Acknowledgements

The authors acknowledge Prof. Aharon Oren at The Hebrew University of Jerusalem for providing useful advice for naming the new bacterium.

Funding

This work was supported by the Fundamental Research Funds for Zhejiang Provincial Universities and Research Institutes (2021J020), the National Natural Science Foundation of China (41876114), the Specific Project for Zhejiang Ocean University from Municipal Science and Technology Bureau of Zhoushan (2022C41018, 20210198), and the Scientific Research Startup Foundation of Zhejiang Ocean University (11104150319/001), and Special Fund for Scientific Innovation Strategy-construction of High Level Academy of Agriculture Science-Distinguished Scholar (2020PY-JC001).

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Authors and Affiliations

  1. Department of Marine Chemistry, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China

    Wen-Zhuo Zhu, Hui-Min Gao & Xiao-Ling Zhang

  2. National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China

    Ya-Ming Ge

  3. Key Laboratory of Fermentation Engineering, Natural “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Ministry of Education, Wuhan, China

    Jun Dai

  4. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China

    Xi Yang

  5. ABI Group, Zhejiang Ocean University, Zhoushan, China

    Xiao-Ling Zhang & Qiao Yang

Authors
  1. Wen-Zhuo Zhu
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  2. Hui-Min Gao
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  4. Jun Dai
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  5. Xi Yang
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  6. Xiao-Ling Zhang
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  7. Qiao Yang
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Contributions

QY and XZ conceived the project. WZ, HG, YG and XY performed the experiments, WZ, HG, YG, JD, and XY analyzed the data, and XZ and QY drafted and revised the manuscript. All authors have read and approved the final version of the manuscript.

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Correspondence to Xiao-Ling Zhang or Qiao Yang.

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Zhu, WZ., Gao, HM., Ge, YM. et al. Alexandriicola marinus gen. nov., sp. nov., a new member of the family Rhodobacteraceae isolated from marine phycosphere. Antonie van Leeuwenhoek 115, 473–486 (2022). https://doi.org/10.1007/s10482-022-01710-2

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