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Bacillus zanthoxyli sp. nov., a novel nematicidal bacterium isolated from Chinese red pepper (Zanthoxylum bungeanum Maxim) leaves in China

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Abstract

A novel strain, 1433T, was isolated from leaves of Chinese red pepper (Huajiao, Zanthoxylum bungeanum Maxim) collected from Gansu province in northwestern China, and was characterised by a polyphasic approach. Cells of strain 1433T were observed to be Gram-stain positive, aerobic, asporogenous, rod shaped, motile and to have peritrichous flagella. The strain was observed to grow at a range of temperatures and pH, 4–45 °C (optimum 28–32 °C) and 6.0–10.0 (optimum pH 6.0–7.0), respectively. Growth was found to occur in the presence of 0–7% (w/v) NaCl [optimum 0–3% (w/v)]. The G+C content of the genomic DNA was determined to be 41.9 mol% and the cell wall peptidoglycan found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7 and the major polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified polar lipid and three unidentified phospholipids. The major cellular fatty acids were identified as iso-C15:0 (31.6%), anteiso-C15:0 (26.9%) and iso-C14:0 (17.1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1433T is a member of the genus Bacillus and is closely related to Bacillus aryabhattai DSM 21047T (99.4% sequence similarity) and Bacillus megaterium DSM 32T (99.2%). DNA–DNA relatedness of the novel strain 1433T with B. aryabhattai DSM 21047T and B. megaterium DSM 32T was 33.8 ± 2.8% and 28.9 ± 3.4%, respectively. On the basis of the polyphasic evidence presented, strain 1433T is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus zanthoxyli sp. nov. The type strain is 1433T (= CCTCC AB 2016326T = KCTC33730T).

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Acknowledgements

The research reported here was supported by projects from the National Basic Research Program of China (2013CB127506), the National Natural Science Foundation Program of China (31200086, 31660023, 31460028, 31570108), and Longnan Municipal Science and Technology Project (2014-08).We are thankful to research assistant Ling-ling Yang for technical assistance and helpful suggestions for revising manuscript.

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Author notes

  1. Ma Li and Cao Yong Hong have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, People’s Republic of China

    Ma Li, Zheng Shuai Chao, Yang Cheng Gang, Duo Jin Ling, Zhou Xing Kui & Mo Ming He

  2. Longnan Economic Forest Research Institute, Longnan, 746000, People’s Republic of China

    Cao Yong Hong

  3. Edible Fungus Research Institute of Hunan Province, Changshan, 410013, People’s Republic of China

    Wang Xiao Yan

  4. Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, 450001, People’s Republic of China

    Xi Jia Qin

  5. Yunnan of CNTC, Kunming, 650202, People’s Republic of China

    Zhu Ming Liang

  6. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Ma Li, Zheng Shuai Chao, Yang Cheng Gang, Duo Jin Ling, Zhou Xing Kui & Mo Ming He

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  1. Ma Li
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Correspondence to Mo Ming He.

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Li, M., Hong, C.Y., Yan, W.X. et al. Bacillus zanthoxyli sp. nov., a novel nematicidal bacterium isolated from Chinese red pepper (Zanthoxylum bungeanum Maxim) leaves in China. Antonie van Leeuwenhoek 110, 1179–1187 (2017). https://doi.org/10.1007/s10482-017-0890-y

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