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Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan

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Abstract

A Gram-positive, moderately halotolerant, rod-shaped, spore forming bacterium, designated strain FJAT-14515T was isolated from a soil sample in Cihu area, Taoyuan County, Taiwan. The strain grew at 10–35 °C (optimum at 30 °C), pH 5.7–9.0 (optimum at pH 7.0) and at salinities of 0–5 % (w/v) NaCl (optimum at 1 % w/v). The diagnostic diamino acid of the peptidoglycan of the isolated strain was meso-diaminopimelic acid and major respiratory isoprenoid quinone was MK-7. Major cellular fatty acids were anteiso-C15:0 (40.6 %), iso-C15:0 (20.7 %) and the DNA G+C content of strain FJAT-14515T was 37.1 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-14515T belongs to the genus Bacillus, and was most closely related to the reference strains of Bacillus muralis DSM 16288T (97.6 %) and Bacillus simplex DSM 1321T (97.5 %). Levels of DNA–DNA relatedness between strain FJAT-14515T and the reference strains of B. muralis DSM 16288T and B. simplex DSM 1321T were 27.9 % ± 3.32 and 44.1 % ± 0.57, respectively. Therefore, on the basis of phenotypic, chemotaxonomic and genotypic properties, strain FJAT-14515T represents a novel species of the genus Bacillus, for which the name Bacillus cihuensis sp. nov. is proposed. The type strain is FJAT-14515T (=DSM 25969T = CGMCC 1.12697T).

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Acknowledgments

We thank Professor J. P. Euzéby for his suggestion on the spelling of the specific epithet. We thank also the Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, PR China, and the international cooperation project of Chinese Ministry of Science and Technology (2012DFA31120), Natural Science Foundation of China (NSFC) (31370059), 948 project of Chinese Ministry of Agriculture (2011-G25), 973 program earlier research project (2011CB111607) and project of agriculture science and technology achievement transformation (2010GB2C400220) for the supporting, respectively.

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Authors and Affiliations

  1. Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China

    Bo Liu, Guo-Hong Liu, Jian-Yang Tang & Mei-Chun Chen

  2. Institute of Crop Sciences and Resource Conservation (INRES), University of Bonn, Meckenheimer Allee 166A, 53115, Bonn, Germany

    Cetin Sengonca

  3. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße7B, 38124, Braunschweig, Germany

    Peter Schumann

  4. Department of Agricultural Chemistry, National Taiwan University, Taipei, 10617, Taiwan

    Ming-Kuang Wang

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  1. Bo Liu
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Correspondence to Bo Liu.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain FJAT-14515T is JX262264.

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Liu, B., Liu, GH., Sengonca, C. et al. Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan. Antonie van Leeuwenhoek 106, 1147–1155 (2014). https://doi.org/10.1007/s10482-014-0284-3

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