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Gordonia jinhuaensis sp. nov., a novel actinobacterium, isolated from a VBNC (viable but non-culturable) state in pharmaceutical wastewater

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Abstract

A Gram-stain positive, aerobic, non-motile and rod-shaped actinobacterial strain, designated as ZYR 51T, was isolated from pharmaceutical wastewater in Jinhua city, Zhejiang province, Eastern China. Isolation was aided by using a resuscitation-promoting factor, suggesting the strain was recovered from a viable but non-culturable state. Strain ZYR 51T was characterized by a polyphasic taxonomic approach. Growth was found to occur at 10–45 °C, pH 6.0–10.0 and 0–9 % NaCl (w/v). Based on the 16S rRNA gene sequence, phylogenetic analysis clearly demonstrated that strain ZYR 51T belongs to the genus Gordonia and showed low level similarities (below 97 %) with all other members of this genus. The strain was found to possess meso-diaminopimelic acid (meso-DAP), along with MK-9(H2) as the predominant menaquonine. Mycolic acids were found to be present. C16:0 (34.9 %), 10-methyl C18:0 (30.3 %), iso-C18:0(8.2 %), and summed feature 3 (C16:1 ω6c and/or C16:1ω7c as define by MIDI; 18.8 %) were identified as the major cellular fatty acids. The polar lipid profile of strain ZYR 51T was found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and some unknown lipids. The genomic DNA G+C content of strain ZYR 51T was determined to be 67.7 mol%. The combined genotypic and phenotypic data showed that the strain represents a novel species of the genus Gordonia, for which the name Gordonia jinhuaensis sp. nov. is proposed, with the type strain is ZYR 51T (=CGMCC 1.12827T = NBRL B-59111T = NBRC 110001T).

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31340071), National Basic Research Program of China (No. 2010CB833801), Key Project of Yunnan Provincial Natural Science Foundation (2013FA004) and a Grant NRF-2006-08790 funded by Ministry of Science, ICT and Future Planning of Korean government. WH and WJL extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project (No. RGP-VPP-205).

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Shan-Hui Li, Juan Cheng & Wen-Jun Li

  2. College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China

    Yi Jin & Lin-Xian Ding

  3. Microbial Resource Center KRIBB, Daejeon, 305-806, Republic of Korea

    Dong-Jin Park & Chang-Jin Kim

  4. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Wen-Sheng Shu & Wen-Jun Li

  5. Bioproducts Research Chair (BRC), College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein & Mohammed A. M. Wadaan

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  1. Shan-Hui Li
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Li, SH., Jin, Y., Cheng, J. et al. Gordonia jinhuaensis sp. nov., a novel actinobacterium, isolated from a VBNC (viable but non-culturable) state in pharmaceutical wastewater. Antonie van Leeuwenhoek 106, 347–356 (2014). https://doi.org/10.1007/s10482-014-0207-3

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