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Halalkalicoccus paucihalophilus sp. nov., a halophilic archaeon from Lop Nur region in Xinjiang, northwest of China

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Abstract

Two extremely halophilic archaea, designated YIM 93701T and YIM 93664, were isolated from Lop Nur region in Xinjiang Province, northwest of China. The cells of the two strains were observed to be cocci, non-motile and Gram-negative. The organisms were determined to be aerobic and required at least 6 % NaCl for growth (optimum 20–25 % and maximum 35 %). Growth was found to occur in the ranges of 16–50 °C (optimum 37 °C) and pH 6.0–8.5 (optimum 6.5–7.5). Cells did not lyse in distilled water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belongs to the genus Halalkalicoccus and possessed 99.3 and 99.5 % similarities with their closest phylogenetic relative Halalkalicoccus tibetensis JCM 11890T. Major polar lipids of the two strains were determined to be phosphatidylglycerol(PG),phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and three unidentified glycolipids. The DNA G+C contents were determined to be 60.0–60.4 mol%. The DNA hybridization between the two strains was 92.0 %. In addition, the hybridizations of both strains to H. tibetensis were 49 and 52 %, respectively, and to Halalkalicoccus jeotali were 38 and 33 %, respectively. On the basis of physiological, biochemical tests and phylogenetic differentiations, strains YIM 93701T and YIM 93664 were classified as the same species which represent a novel species in the genus Halalkalicoccus, for which the name Halalkalicoccus paucihalophilus sp. nov. is proposed. The type strain is YIM 93701T (=JCM 17505T = CCTCC 2012803T).

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Acknowledgments

We are grateful to Dr. Heng-Lin Cui for his kindly providing the type strains and valuable comments on our experiments. This research was supported by National Natural Science Foundation of China (No. 31200008), National New Drug Research and Development Project (No. 2010ZX09401-403) and 863 projects (No. 2012AA021705). Y.-G. Zhang wishes to thank the West Light Foundation of The Chinese Academy of Sciences. W.-J. Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, 830011, Ürűmqi, People’s Republic of China

    Bing-Bing Liu, Yong-Guang Zhang, Li Li, Yuan-Ming Zhang & Wen-Jun Li

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Bing-Bing Liu, Shu-Kun Tang, Juan Cheng & Wen-Jun Li

  3. The National Engineering Center for Research of Microbial Pharmaceuticals and New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Shijiazhuang, 050015, People’s Republic of China

    Xin-Hua Lu

  4. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar, 843300, People’s Republic of China

    Li-Li Zhang

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  1. Bing-Bing Liu
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Correspondence to Wen-Jun Li.

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Bing-Bing Liu and Shu-Kun Tang contributed equally to this study.

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Liu, BB., Tang, SK., Zhang, YG. et al. Halalkalicoccus paucihalophilus sp. nov., a halophilic archaeon from Lop Nur region in Xinjiang, northwest of China. Antonie van Leeuwenhoek 103, 1007–1014 (2013). https://doi.org/10.1007/s10482-013-9880-x

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