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Thermoactinospora rubra gen. nov., sp. nov., a thermophilic actinomycete isolated from Tengchong, Yunnan province, south–west China

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Abstract

Two novel Gram-positive, spore-forming, thermophilic actinomycetes, designated as strain YIM 77501T and YIM 77570, were isolated from a sandy soil sample collected at Tengchong National Volcanic Geological Park, Yunnan province, south–west China. Phylogenetic analysis based on the 16S rRNA gene sequences suggested that the two isolates fell within the family Streptosporangiaceae. The strains formed extensively branched substrate and aerial mycelia which carried masses of long, straight or irregular spore chains composed of warty ornamented spores. Cell walls of the two strains contained meso-diaminopimelic acid and glucose, galactose, mannose and ribose were detected as whole-cell sugars. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids and phosphatidylinositol, phosphatidylinositolmannosides. The major cellular fatty acids were iso-C16:0 and 10-methyl C17:0. The DNA G+C content was 74–76 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as the phylogenetic analysis, these strains represents a novel species of a new genus within the family Streptosporangiaceae, for which the name Thermoactinospora rubra gen. nov., sp. nov. is proposed. The type strain of T. rubra is YIM 77501T (=DSM 45614T = CCTCC AA 2011014T).

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31070007) and International Cooperation Research Program of Yunnan Province (No. 2009AC017). Special thanks should be given to the entire staff from Tengchong National Volcanic Geological Park for their help and support during the field sampling.

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    En-Min Zhou, Shu-Kun Tang, Zhao-Qi Song, Tian-Tian Yu, Ling-Ling Yang & Wen-Jun Li

  2. Novozymes A/S, Krogshoejvej, 2880, Bagsvaerd, Denmark

    Carsten Sjøholm

  3. Engineering Technology Research Center of Biomass Degradation and Gas-Forming, Shangqiu Normal University, Shangqiu, 476000, People’s Republic of China

    Zhao-Qi Song

  4. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

  5. College of Life Science, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Guo-Xing Nie

  6. Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

    Wen-Jun Li

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Zhou, EM., Tang, SK., Sjøholm, C. et al. Thermoactinospora rubra gen. nov., sp. nov., a thermophilic actinomycete isolated from Tengchong, Yunnan province, south–west China. Antonie van Leeuwenhoek 102, 177–185 (2012). https://doi.org/10.1007/s10482-012-9725-z

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