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Streptomonospora tuzyakensis sp. nov., a halophilic actinomycete isolated from saline soil

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Abstract

A novel actinobacterium, designated strain BN506T, was isolated from soil collected from Tuz (Salt) Lake, Konya, Turkey, and was characterised to determine its taxonomic position. The isolate was found to have morphological and chemotaxonomic properties associated with members of the genus Streptomonospora. The isolate was found to grow optimally at 37 °C and in the presence of 10 % (w/v) NaCl but not in the absence of NaCl. Phylogenetic analyses based on an almost-complete 16S rRNA gene sequences indicated that isolate is closely related to members of the genus Streptomonospora and forms a distinct phyletic line in the Streptomonospora phylogenetic tree. Strain BN506T is closely related to Streptomonospora halophila YIM 91355T (98.1 % sequence similarity). Sequence similarities with other type strains of the genus Streptomyces were lower than 98.0 %. The cell wall of the novel strain was found to contain meso-diaminopimelic acid. Whole cell hydrolysates were found to contain galactose, glucose and ribose. The predominant menaquinone was identified as MK-10(H8) (57.0 %). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and 10 methyl C18:0. Based on 16S rRNA gene sequence analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain BN506T was identified as a member of a novel species of the genus Streptomonospora, for which the name Streptomonospora tuzyakensis sp. nov. (type strain BN506T = DSM 45930T = KCTC 29210T) is proposed.

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Acknowledgments

This research was supported by Ondokuz Mayis University (OMU), project no. PYO. FEN. 1901.11.011.

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Authors and Affiliations

  1. Biology Department, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey

    Demet Tatar & Nevzat Sahin

  2. Biology Department, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey

    Kiymet Guven

  3. Molecular Biology and Genetics Department, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Kadriye Inan

  4. Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey

    Demet Cetin

  5. Biology Department, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Ali Osman Belduz

Authors
  1. Demet Tatar
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  2. Kiymet Guven
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  3. Kadriye Inan
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  4. Demet Cetin
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  5. Ali Osman Belduz
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  6. Nevzat Sahin
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Corresponding author

Correspondence to Nevzat Sahin.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of Streptomonospora tuzyakensis BN506T(=DSM 45930T = KCTC 29210T) is KC959224.

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10482_2015_607_MOESM1_ESM.jpg

Scanning electron micrograph of strain BN506T grow on ISP 2 (Shirling and Gottlieb 1966) medium with 10 % NaCl for 30 days at 37 °C showing spore chains (a) and single spore (b). Bars, 1 μm. (JPEG 32 kb)

10482_2015_607_MOESM2_ESM.tif

Molybdophosporic acid stained two-dimensional TLC of polar lipids from strain BN506T. DPG: diphosphatidylglycerol, PG: phosphatidylglycerol, PC: phosphatidylcholine, GL: 1-3 three unidentified glycolipids, PL: unidentified phospholipid. (TIFF 2271 kb)

10482_2015_607_MOESM3_ESM.doc

Maximum parsimony tree based on almost complete 16S rRNA gene sequences showing the position of strain BN506T amongst its phylogenetic neighbours. Streptosporangium album DSM 43023T was used as an outgroup. Numbers at the nodes indicate the levels of bootstrap support (%); only values ≥ 50 % are shown. GenBank accession numbers are given in parentheses. Maximum likelihood tree based on almost complete 16S rRNA gene sequences showing the position of strain. BN506T amongst its phylogenetic neighbours. Streptosporangium album DSM 43023T was used as an outgroup. Numbers at the nodes indicate the levels of bootstrap support (%); only values ≥ 50 % are shown. GenBank accession numbers are given in parentheses. Bar, 0.01 substitutions per site. (DOC 106 kb)

Fatty acid profiles of strain BN506T and closely related type strains. (DOC 40 kb)

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Tatar, D., Guven, K., Inan, K. et al. Streptomonospora tuzyakensis sp. nov., a halophilic actinomycete isolated from saline soil. Antonie van Leeuwenhoek 109, 35–41 (2016). https://doi.org/10.1007/s10482-015-0607-z

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