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Tyramine reduction by tyrosine decarboxylase inhibitor in Enterococcus faecium for tyramine controlled cheonggukjang

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Abstract

This study was carried out to find a method to control tyrosine decarboxylase activity (TDC) of a strain of Enterococcus faecium capable of producing high levels of tyramine. To select a TDC inhibitor, enzyme assay was first performed using purified TDC enzyme and 0.1% of TDC inhibiting chemicals. When 0.23% of nicotinic acid was added, tyramine content (363 ug/mL) was lower than that of the control group (873 ug/mL). At the same time, bacterial growth was decreased 1 log cycle from 8.62 to 7.56 log CFU/mL. TDC expression level in E. faecium was measured by using RT-qPCR. Lower expression level (below 0.7) was observed after the addition of 0.23% nicotinic acid (in vitro). When cheonggukjang was manufactured with addition of nicotinic acid, tyramine contents were decreased from 698.67 to 117.27 mg/kg when the concentration of nicotinic acid added was increased from 0.10 to 0.30%. These results suggest that nicotinic acid could be used as an agent (TDC inhibitor) to reduce tyramine content in cheonggukjang.

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Acknowledgements

This research was supported by the High Value-added Food Technology Development Program (314057-032-SB020) funded by Korea Institute of Planning and Evaluation for Technology in Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

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Authors and Affiliations

  1. Department of Food and Biotechnology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 339-700, Korea

    Hyang-Rin Kang, Ho-Sik Kim, Jae-Hyung Mah, Young-Wan Kim & Han-Joon Hwang

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  1. Hyang-Rin Kang
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  2. Ho-Sik Kim
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  3. Jae-Hyung Mah
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  4. Young-Wan Kim
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  5. Han-Joon Hwang
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Correspondence to Han-Joon Hwang.

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Kang, HR., Kim, HS., Mah, JH. et al. Tyramine reduction by tyrosine decarboxylase inhibitor in Enterococcus faecium for tyramine controlled cheonggukjang . Food Sci Biotechnol 27, 87–93 (2018). https://doi.org/10.1007/s10068-017-0205-0

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  • DOI: https://doi.org/10.1007/s10068-017-0205-0

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