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Isolation and functional characterization of 3-phosphoglycerate dehydrogenase involved in salt responses in sugar beet

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Abstract

The present study investigated the significance of serine biosynthetic genes for salt stress in sugar beet (Beta vulgaris). We isolated a total of four genes, two each encoding D-3-phosphoglycerate dehydrogenase (BvPGDHa and BvPGDHb) and serine hydroxymethyl transferase (BvSHMTa and BvSHMTb). mRNA transcriptional expression for BvPGDHa was significantly enhanced under salt stress conditions in both leaves and roots of sugar beet, whereas it was reduced for BvPGDHb. On the other hand, BvSHMTa was expressed transiently in leaves and roots under salt stress, whereas expression level of BvSHMTb was not altered. PGDH activity was high in storage root. After salt stress, PGDH activity was increased in leaf, petiole, and root. Recombinant proteins were expressed in Escherichia coli. The K m values for 3-phosphoglycerate in PGDHa and PGDHb were 1.38 and 2.92 mM, respectively. The findings suggest that BvPGDHa and BvSHMTa play an important role during salt stress in sugar beet.

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Abbreviations

BADH:

Betaine aldehyde dehydrogenase

CMO:

Choline monooxygenase

3-PGA:

D-3-phosphoglycerate

PGDH:

D-3-phosphoglycerate dehydrogenase

PHP:

Phosphohydroxypyruvate

PSAT:

Phosphoserine aminotransferase

PSP:

Phosphoserine phosphatase

RuBisCO:

Ribulose-1,5-bisphosphatase carboxylase/oxygenase

SHMT:

Serine hydroxymethyl transferase

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Acknowledgements

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan; Salt Science Research Foundation; and the International Center for Natural Environmental Science of Meijo University, Japan.

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Authors and Affiliations

  1. Graduate School of Environmental and Human Sciences, Meijo University, Nagoya, 468-8502, Japan

    Kunihide Kito, Shota Sakakibara, Yoshito Tanaka & Teruhiro Takabe

  2. Research Institute, Meijo University, Tenpaku-ku, Nagoya, Aichi, 468-8502, Japan

    Koichi Tsutsumi, Nana Yamada-Kato & Teruhiro Takabe

  3. National Research Center on Plant Biotechnology IARI, New Delhi, 110012, India

    Vandna Rai

  4. National Center for Genetic Engineering and Biotechnology, 113 Paholyothin Rd., Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand

    Cattarin Theerawitaya & Suriyan Cha-um

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  1. Kunihide Kito
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  2. Koichi Tsutsumi
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  3. Vandna Rai
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  4. Cattarin Theerawitaya
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  9. Teruhiro Takabe
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Correspondence to Teruhiro Takabe.

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The authors declare that they have no conflict of interest.

Additional information

Handling Editor: Bhumi Nath Tripathi

Note that nucleotide sequence data for the D-3-phosphoglycerate dehydrogenase genes, BvPGDHa and BvPGDHb, and serine hydroxymethyl transferase genes, BvSHMTa and BvSHMTb, from sugar beet are available in the DDJB database under the accession numbers LC201809, LC201810, LC201811 and LC201812, respectively.

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Kito, K., Tsutsumi, K., Rai, V. et al. Isolation and functional characterization of 3-phosphoglycerate dehydrogenase involved in salt responses in sugar beet. Protoplasma 254, 2305–2313 (2017). https://doi.org/10.1007/s00709-017-1127-7

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  • DOI: https://doi.org/10.1007/s00709-017-1127-7

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