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Grass carp reovirus outer capsid proteins VP5 and VP7 interact in vitro

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Abstract

Grass carp reovirus (GCRV) causes viral hemorrhagic disease in cultured grass carp. However, there is no effective means of controlling GCRV. Previous studies have shown, by cryoelectron microscopy, that the outer capsid of GCRV is composed of 200 trimers of VP5-VP7 heterodimers. However, confirmation of this interaction between VP5 and VP7 through molecular biochemistry is still lacking. This study characterized the interactions between VP5 and VP7 in vitro. VP5 was shown to interact with VP7 in a commercial yeast-two-hybrid screen. A dot-blot overlay assay was used to show that VP7 binding to VP5 was dose-dependent. Finally, a yeast-two-hybrid approach confirmed interactions between full-length and truncated forms of VP5 and VP7. In conclusion, our results indicate that VP5 and VP7 interact directly in vitro.

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Acknowledgements

This work was supported by Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, 201306 Shanghai, China.

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  1. Weisha Liu and Hao Wang contribute equally to this paper.

Authors and Affiliations

  1. Key Laboratory of Freshwater Fishery Germplasm Resources, National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, 201306, China

    Weisha Liu, Hao Wang, Fei Yu & Liqun Lu

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  1. Weisha Liu
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  2. Hao Wang
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  3. Fei Yu
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  4. Liqun Lu
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Contributions

Conceived and designed the experiments: LL, HW. Performed the experiments: HW, WSL. Analyzed the data: HW, WSL, LL. Wrote the paper: HW, LL.

Corresponding author

Correspondence to Liqun Lu.

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Funding

This work was supported by grants from the Earmarked Fund for China Agriculture Research System (No. CARS-46-12) and the National Science Foundation of China (No. 31672690, No. 31072244).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Liu, W., Wang, H., Yu, F. et al. Grass carp reovirus outer capsid proteins VP5 and VP7 interact in vitro . Arch Virol 162, 2375–2380 (2017). https://doi.org/10.1007/s00705-017-3354-9

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  • DOI: https://doi.org/10.1007/s00705-017-3354-9

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