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Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus

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Abstract

P6 of southern rice black-streaked dwarf virus (SRBSDV) is a multifunctional protein that is involved in the formation of viroplasms by interacting with P5-1. Here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between SRBSDV P6 and P9-1 in yeast and plant cells. Mutational analysis showed that the N-terminal region (residues 1–93) of P6 was necessary for the interaction between P6 and P9-1. Self-interactions only occurred between the full-length P6 or P9-1. P9-1 was able to form viroplasm-like inclusion structures alone in the absence of other viral proteins.

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Acknowledgments

This work was funded by National Science and Technology Support Program (2012BAD19B03), the China 973 Program (2010CB126203), the Special Fund for Agro-Scientific Research in the Public Interest of China (201003031), 863 Program (2007AA10Z414), the Zhejiang Provincial Science and Technology Project (2010C12027), and the Zhejiang Provincial Foundation for Natural Science (LQ14C140003). We thank Professor M. J. Adams, Stevenage, UK, for help in correcting the English of the manuscript.

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Authors and Affiliations

  1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Jing Li, Jin Xue, Heng-Mu Zhang, Jian Yang, Li Xie & Jian-Ping Chen

  2. College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, 410128, China

    Jin Xue

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  1. Jing Li
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  2. Jin Xue
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  6. Jian-Ping Chen
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Correspondence to Heng-Mu Zhang or Jian-Ping Chen.

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Li, J., Xue, J., Zhang, HM. et al. Characterization of homologous and heterologous interactions between viroplasm proteins P6 and P9-1 of the fijivirus southern rice black-streaked dwarf virus. Arch Virol 160, 453–457 (2015). https://doi.org/10.1007/s00705-014-2268-z

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  • DOI: https://doi.org/10.1007/s00705-014-2268-z

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