Abstract
Solanum nodiflorum mottle virus (SNMoV) was isolated from a small-flowered nightshade (Solanum nodiflorum) in Queensland, Australia. It has been included in the genus Sobemovirus based on virion morphology and serological relationships. Here, we report the sequence of the complete genome of SNMoV. Sequence analysis confirmed that SNMoV has the characteristic genome organization of sobemoviruses. Phylogenetic analysis showed that it clusters most closely with velvet tobacco mottle virus (VTMoV), another sobemovirus native to Australia. Their genomes show 56.8 % sequence identity.
References
Greber RS, Randles JW (1986) Solanum nodiflorum mottle virus. In: Descriptions of plant viruses, no. 318. CMI/AAB
Truve E, Fargette D (2012) Genus Sobemovirus. In: King AMQ, Carstens E, Adams M, Lefkowitz E (eds) Virus taxonomy. Elsevier, Ninth report of the international committee on taxonomy of viruses, pp 1185–1189
Randles JW, Davies C, Hatta T, Gould AR, Francki RIB (1981) Studies on encapsidated viroid-like RNA. I. Characterization of Velvet tobacco mottle virus. Virology 108:111–122
Chu PWG, Francki RIB (1983) Chemical and serological comparison of the coat proteins of velvet tobacco mottle and Solanum nodiflorum mottle viruses. Virology 129:350–356
Jones AT, Mayo MA (1984) Satellite nature of the viroid-like RNA-2 Solanum nodiflorum mottle virus and the ability of other plant viruses to support the replication of viroid-like RNA molecules. J Gen Virol 65:1713–1721
Francki RIB, Grivell CJ, Gibb KS (1986) Isolation of velvet tobacco mottle virus capable of replication with and without a viroid-like RNA. Virology 148:381–384
Haseloff J, Simons RH (1982) Comparative sequence and structure of viroid-like RNA-s of two plant viruses. NAR 10:3681–3691
Arthur K, Dogra S, Randles JW (2010) Complete nucleotide sequence of Velvet tobacco mottle virus isolate K1. Arch Virol 155:1893–1896
Oñate-Sánchez L, Vicente-Carbajosa J (2008) DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques. BMC Res Notes 1:93
Tars K, Zeltiņš A, Liljas L (2003) The three-dimensional structure of cocksfoot mottle virus at 2.7 Å resolution. Virology 310:287–297
Sõmera M, Truve E (2015) Rottboellia yellow mottle virus is a distinct species in the genus Sobemovirus. Arch Virol 160:857–863
Ling R, Pate AR, Carr JP, Firth AE (2013) An essential fifth coding ORF in the sobemoviruses. Virology 446:397–408
Gouy M, Guindon S, Gascuel O (2010) Seaview version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224
Sõmera M, Sarmiento C, Truve E (2015) Overview on sobemoviruses and a proposal for the creation of the family Sobemoviridae. Viruses 7:3076–3115
Siré C, Bangratz-Reyser M, Fargette D, Brugidou C (2008) Genetic diversity and silencing suppression effects of Rice yellow mottle virus and the P1 protein. Virol J 5:55–62
Gillet FX, Cattoni DI, Petiot-Becard S, Delalande F, Poignavent V, Brizard JP, Bessin Y, Dorsselaer AV, Declerck N, Sanglier-Cianferani S, Brugidou C, Vignols S (2013) The RYMV-encoded viral suppressor of RNA silencing P1 is a zinc-binding protein with redox-dependent flexibility. J Mol Biol 425:2423–2435
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation protein database search programs. Nucleic Acid Res 25:3389–3402
Sõmera M, Truve E (2013) The genome organization of lucerne transient streak and turnip rosette sobemoviruses revisited. Arch Virol 158:673–678
Nair S, Savithri HS (2010) Natively unfolded nucleic acid binding P8 domain of SeMV polyprotein 2a affects the novel ATPase activity of the preceding P10 domain. FEBS Lett 584:571–576
Govind K, Mäkinen K, Savithri HS (2012) Sesbania mosaic (SeMV) infectious clone: possible mechanism of 3’ and 5’ end virus repair and role of polyprotein processing in viral replication. PLoS One 7:e31190
Mäkinen K, Mäkeläinen K, Arshava N, Tamm T, Merits A, Truve E, Zavriev S, Saarma M (2000) Characterization of VPg and the polyprotein processing of cocksfoot mottle virus (genus Sobemovirus). J Gen Virol 81:2783–2789
Nair S, Savithri HS (2010) Processing of SeMV polyproteins revisited. Virology 396:106–117
Olspert A, Peil L, Hébrard E, Fargette D, Truve E (2011) Protein-RNA linkage and post-translational modifications of two sobemovirus VPgs. J Gen Virol 92:445–452
Tamm T, Suurväli J, Lucchesi Y, Olspert A, Truve E (2009) Stem-loop structure of cocksfoot mottle virus RNAis indispensable for programmed-1 ribosomal frameshifting. Virus Res 146:73–80
Hollings M, Stone OM, Barton RJ, Greber RS (1979) Solanum nodiflorum virus. Ann Rep Glasshouse Crops Res Inst 1978:150–151
Kiberstis PA, Zimmern D (1984) Translational strategy of Solanum nodiflorum mottle virus RNA: synthesis of a coat protein precursor in vitro and in vivo. NAR 12:933–943
Lee SK, Hacker DL (2001) In vitro analysis of an RNA binding site within the N-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. Virology 286:317–327
Satheshkumar PS, Lokesh GL, Murthy MRN, Savithri HS (2005) The role of arginine-rich motif and β-annulus in the assembly and stability of sesbania mosaic virus capsids. J Mol Biol 353:447–458
Olspert A, Paves H, Toomela R, Tamm T, Truve E (2010) Cocksfoot mottle sobemovirus coat protein contains two nuclear localization signals. Virus Genes 40:423–431
Plevka P, Tars K, Zeltiņš A, Balke I, Truve E, Liljas L (2007) The three-dimensional structure of Ryegrass mottle virus at 2.9 Ǻ resolution. Virology 369:364–374
Acknowledgements
S. nodiflorum and S. chenopodioides seeds were kindly distributed by IPK-Gatersleben. We appreciate Dr. Ulrike Lohwasser for the identification of Solanum species, and Signe Nõu for excellent plant care.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Funding
This study was funded by institutional research fund IUT19-3 of the Estonian Ministry of Education and Research and by Estonian Research Council Grant No. 9415.
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with animal or human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Sõmera, M., Truve, E. Complete nucleotide sequence of Solanum nodiflorum mottle virus. Arch Virol 162, 1731–1736 (2017). https://doi.org/10.1007/s00705-017-3273-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-017-3273-9