Abstract
Velvet tobacco mottle virus (VTMoV) infects the native Australian plant Nicotiana velutina, which is endemic to central Australia. This virus is included in the genus Sobemovirus based on virion morphology and serological relationships. We report here the full genome sequence of VTMoV, attained using a genome-walking strategy with both degenerate and specific primers. This sequence confirms that VTMoV is a sobemovirus, with the same open reading frame (ORF) organisation as other described sobemoviruses. The VTMoV sequence is closest to those sobemoviruses isolated from monocotyledonous plants, although the narrow host range of VTMoV is limited to dicotyledonous plants.
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Acknowledgments
This work was funded by the C.J. Everard Postgraduate Scholarship to KA. We thank Dr. Allan Gould, University of Queensland, for supplying his anonymous degenerate primers and Dr. Nick Collins for advice on the RACE protocol.
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The authors declare that they have no conflict of interest.
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The sequence reported here has NCBI GenBank accession number HM754263.
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Arthur, K., Dogra, S. & Randles, J.W. Complete nucleotide sequence of Velvet tobacco mottle virus isolate K1. Arch Virol 155, 1893–1896 (2010). https://doi.org/10.1007/s00705-010-0801-2
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DOI: https://doi.org/10.1007/s00705-010-0801-2