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A two-wavelength fluorescence recovery method for the simultaneous determination of aureomycin and oxytetracycline by using gold nanocrystals modified with serine and 11-mercaptoundecanoic acid

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Abstract

A method is described for rapid (1 h) synthesis of gold nanoclusters (AuNCs) co-functionalized with serine and 11-mercaptoundecanoic acid. The co-functionalized AuNCs exhibit good stability towards temperature, pH values, and over time. They were characterized by atomic force microscopy, high-resolution transmission electron microscopy, dynamic light scattering, and by fluorescence, IR and X-ray photoelectron spectroscopies. The fluorescence of the AuNCs is quenched by Hg(II) and restored on subsequent addition of aureomycin (CTC) or oxytetracycline (OTC). A fluorescent turn-on assay was worked out for simultaneous detection of CTC and OTC based on recording the change of the restored fluorescence measured at 420 and 500 nm under 340 nm photoexcitation. The detection limits are 20 and 9 nM for CTC and OTC, respectively. The concentrations of CTC and OTC can also be visualized by UV illumination. The nanoprobe was successfully applied to the simultaneous determination of CTC and OTC in spiked human urine.

Schematic of a two-wavelength fluorescence recovery method for the simultaneous determination of aureomycin and oxytetracycline. It is based on the use of gold nanocrystals modified with serine and 11-mercaptoundecanoic acid, and on recording the change of the restored fluorescence measured at 420 and 500 nm.

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Acknowledgements

S. Xu, Y. Mao, Y. Nie and J. Wang are grateful for the financialsupport provided by NSFC (21505081) and the Doctoral Found of QUST (010022832). X. Luo acknowledges the financial support by NSFC (21422504 and 21675093), the Natural Science Foundation of Shandong Province of China (JQ201406) and the Taishan Scholar Program of Shandong Province, China(ts20110829).

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Authors and Affiliations

  1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Yaning Mao, Yufeng Wu, Yongyin Nie, Jun Wang, Yuanyuan Liu, Shenghao Xu & Xiliang Luo

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  1. Yaning Mao
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  2. Yufeng Wu
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  3. Yongyin Nie
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  4. Jun Wang
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  5. Yuanyuan Liu
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  6. Shenghao Xu
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  7. Xiliang Luo
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Correspondence to Shenghao Xu or Xiliang Luo.

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Mao, Y., Wu, Y., Nie, Y. et al. A two-wavelength fluorescence recovery method for the simultaneous determination of aureomycin and oxytetracycline by using gold nanocrystals modified with serine and 11-mercaptoundecanoic acid. Microchim Acta 185, 222 (2018). https://doi.org/10.1007/s00604-018-2762-1

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