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Association between oestrogen receptor β immunoexpression and cause of culling, ovarian appearance and the existence of PRRS virus in the porcine ovary

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Abstract

The objective of this study was to determine the immunoexpression of oestrogen receptor beta (ERβ) in the gilt ovary and its association with cause of culling, ovarian appearance and the existence of porcine reproductive and respiratory syndrome (PRRS) virus in the ovary. In total, 62 ovaries were collected from culled replacement gilts. The gilts were classified according to the cause of culling (abnormal vaginal discharge, abortion, anoestrus, repeated service and non-reproductive causes), ovarian appearance (non-cycling and cycling) and the existence of PRRS virus in the ovary (not exist and exist). Ovarian ERβ expression was determined using immunohistochemistry. The proportion of ERβ immunostaining in the theca cells surrounding the follicles was evaluated using imaging software. The result revealed that ERβ immunostaining expressed in the theca interna cells of the follicles in the gilt ovary. The ERβ immunostaining was detected in 23.7 ± 19.8% (range 0–98.4%) of the theca cells. The gilts culled due to anoestrus had lower ERβ immunostaining (19.3%) compared to those culled due to non-reproductive causes (28.9%, P = 0.010) and repeated service (31.3%, P = 0.008). However, the expression of ERβ in the ovarian tissues with and without PRRS virus existence was not different (26.8 and 25.2%, respectively, P = 0.538). Likewise, ERβ immunoexpression in the follicles of non-cycling and cycling ovaries was not different (25.8 versus 26.3%, P = 0.851). In conclusion, the gilts culled due to anoestrus had relatively poor ovarian ERβ immunoexpression. Neither the existence of PRRS virus nor ovarian appearance was associated with ERβ immunoexpression in the gilt ovary.

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Acknowledgements

Financial support for the study was provided by a Grant for International Research Integration: Chula Research Scholar, Ratchadaphiseksomphot Endowment Fund. D. Phoophitphong is a grantee of the Royal Golden Jubilee (RGJ) Ph.D. Program, the Thailand Research Fund.

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Authors and Affiliations

  1. Department of Obstetrics, Gynaecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Padet Tummaruk, Amonrat Choonnasard, Nattanicha Prayoonwiwat, Pinya Wuttiwongtanakorn, Chakrit Butrak & Duangkamol Phoophitphong

  2. Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, 10900, Thailand

    Duangkamol Phoophitphong

  3. Department of Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Sayamon Srisuwatanasagul

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  1. Padet Tummaruk
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  2. Amonrat Choonnasard
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  3. Nattanicha Prayoonwiwat
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  4. Pinya Wuttiwongtanakorn
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  6. Duangkamol Phoophitphong
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  7. Sayamon Srisuwatanasagul
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Correspondence to Padet Tummaruk.

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All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

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Tummaruk, P., Choonnasard, A., Prayoonwiwat, N. et al. Association between oestrogen receptor β immunoexpression and cause of culling, ovarian appearance and the existence of PRRS virus in the porcine ovary. Comp Clin Pathol 26, 1049–1055 (2017). https://doi.org/10.1007/s00580-017-2483-6

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  • DOI: https://doi.org/10.1007/s00580-017-2483-6

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