Skip to main content
SpringerLink
Log in

Expression of oestrogen receptor α in the endometrium of porcine reproductive and respiratory syndrome virus-infected gilts

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Porcine reproductive and respiratory syndrome (PRRS) virus infection often lead to infertility in gilts and sows. Nevertheless, the impact of PRRS virus on the endometrial function has not been fully elucidated. The present study aimed to determine the effect of PRRS virus infection on the expression of oestrogen receptor (ER) α in the endometrium of gilts. Uterine tissues from 54 gilts were classified into two groups according to PRRS virus detection using immunohistochemistry (26 positive and 28 negative samples). The reproductive status was classified as prepubertal, luteal and follicular phases. The expression of ERα in the epithelium, subepithelium and glandular tissue layers of the endometrium was determined by immunohistochemistry. ERα immunostaining was detected in 22.2, 13.5 and 33.6 % of the cells in the epithelial, subepithelial and glandular tissue layers, respectively. The ERα immunostaining in the glandular layer of the endometrium in follicular-phase gilts was higher than that in prepubertal gilts (46.1 and 15.2 %, respectively, P = 0.011). The ERα immunostaining in the glandular layer of the endometrium was positively correlated with body weight (r = 0.138, P = 0.020) and average daily gain (r = 0.169, P = 0.005) of the gilts. The ERα immunostaining in all tissue layers of the endometrium with PRRS virus detection did not differ significantly compared to that without PRRS virus (P > 0.05). However, in prepubertal gilts, the ERα immunostaining cells in the epithelial layer of PRRS virus negative (9.5 %) tended to be lower than PRRS virus positive (26.3 %, P = 0.196) uterine tissues. This tendency indicates some impact of PRRS virus infection on the ERα expression in prepubertal gilts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Chastant-Maillard S (2015) Impact of bovine herpesvirus 4 (BoHV-4) on reproduction. Transbound Emerg Dis 62:245–251

    Article  CAS  PubMed  Google Scholar 

  • de Ziegler D, Bulletti C, Fanchin R, Epiney M, Brioschi PA (2001) Contractility of the nonpregnant uterus: the follicular phase. Ann N Y Acad Sci 943:172–184

    Article  PubMed  Google Scholar 

  • Donofrio G, Ravanetti L, Cavirani S, Herath S, Capocefalo A, Sheldon LM (2008) Bacterial infection of endometrial stroma cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease. Reproduction 136:361–366

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jantafong T, Sangtong P, Saenglub W, Mungkundar C, Romlamduan N, Lekchareonsuk C, Lekcharoensuk P (2015) Genetic diversity of porcine reproductive and respiratory syndrome virus in Thailand and Southeast Asia from 2008 to 2013. Vet Microbiol 176:229–238

    Article  CAS  PubMed  Google Scholar 

  • Karniychuk UU, Saha D, Geldhof M, Vanhee M, Cornillie P, van den Breock W, Nauwynck HJ (2011) Porcine reproductive and respiratory syndrome virus (PRRSV) causes apoptosis during its replication in fetal implantation sites. Microb Pathog 51:194–202

    Article  CAS  PubMed  Google Scholar 

  • Mengeling WL, Lager KM, Vorvald CA (1998) Clinical consequences of exposing pregnant gilts to strains of porcine reproductive and respiratory syndrome (PRRS) virus isolated from field cases of “atypical” PRRS. Am J Vet Res 59:1540–1544

    CAS  PubMed  Google Scholar 

  • Olanratmanee E, Wangnaitham S, Thanawongnuwech R, Kunavongkrit A, Tummaruk P (2011) Prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterine tissues in gilts culled due to reproductive disturbance in Thailand. Trop Anim Health Prod 43:451–457

    Article  PubMed  Google Scholar 

  • Olanratmanee E, Nuntawan Na Ayudhya S, Thanawongnuwech R, Kunavongkrit A, Tummaruk P (2013) Reproductive parameters following a PRRS outbreak where a whole-herd PRRS MLV vaccination strategy was instituted post-outbreak. Trop Anim Health Prod 45:1099–1106

    Article  PubMed  Google Scholar 

  • Roongsitthichai A, Cheuchuchart P, Chatwijitkul S, Chantarothai O, Tummaruk P (2013) Influence of age at first estrus, body weight, and average daily gain of replacement gilts on their subsequent reproductive performance as sows. Livest Sci 151:238–245

    Article  Google Scholar 

  • Srisuwatanasagul S (2011) Steroid receptor and their roles in pig uterus. Thai J Vet Med 41(supplement 1):43–49

    Google Scholar 

  • Srisuwatanasagul S, Tummaruk P, Kunavongkrit A (2010) Studies of oestrogen and progesterone receptors in reproductive organs of prepubertal gilts with reproductive disturbance. Thai J Vet Med 40:15–24

    Google Scholar 

  • Sukjumlong S, Kaoeket K, Dalin A-M, Persson E (2003) Immunohistochemical studies on oestrogen receptor alpha (ER∞) and the proliferative marker Ki-67 in the sow uterus at different stages of the oestrus cycle. Reprod Domest Anim 38:5–12

    Article  CAS  PubMed  Google Scholar 

  • Sukjumlong S, Persson E, Kaeoket K, Dalin A-M (2004) Immunohistochemical studies on oestrogen receptor alpha (ER∞) and the proliferative marker Ki-67 in the sow uterus at oestrus and early pregnancy. Reprod Domest Anim 39:361–369

    Article  CAS  PubMed  Google Scholar 

  • Sur JH, Doster AR, Galeota JA, Osorio FA (2001) Evidence for the localization of porcine reproductive and respiratory syndrome virus (PRRSV) antigen and RNA in ovarian follicles in gilts. Vet Pathol 38:58–66

    Article  CAS  PubMed  Google Scholar 

  • Teamsuwan Y, Kaeoket K, Tienthai P, Tummaruk P (2010) Morphological changes and infiltration of immune cells in the endometrium of anoestrus gilt in relation to the ovarian appearance and serum progesterone. Thai J Vet Med 40:31–40

    Google Scholar 

  • Tummaruk P (2012) Effects of season, outdoor climate and photo period on age at first observed estrus in Landrace X Yorkshire crossbred gilts in Thailand. Livest Sci 144:163–172

    Article  Google Scholar 

  • Tummaruk P, Tantilertcharoen R (2012) Seroprevalence of porcine reproductive and respiratory syndrome, Aujeszky’s disease and porcine parvovirus in replacement gilts in Thailand. Trop Anim Health Prod 44:983–989

    Article  PubMed  Google Scholar 

  • Tummaruk P, Kesdangsakonwut S, Kunavongkrit A (2009) Relationships among specific reason for culling, reproductive data and gross-morphology of the genital tracts in gilts culled due to reproductive failure in Thailand. Theriogenology 71:369–375

    Article  CAS  PubMed  Google Scholar 

  • Tummaruk P, Kesdangsakonwut S, Prapasarakul N, Kaeoket K (2010a) Endometritis in gilts: reproductive data, bacterial culture, histopathology, and infiltration of immune cells in the endometrium. Comp Clin Pathol 19:575–584

    Article  Google Scholar 

  • Tummaruk P, Tantasuparuk W, Techakumphu M, Kunavongkrit A (2010b) Influence of repeat-service and weaning-to-first-service interval on farrowing proportion of gilts and sows. Prev Vet Med 96:194–200

    Article  PubMed  Google Scholar 

  • Tummaruk P, Phoophitphong D, Olanratmanee E, Thanawongnuwech R (2015) Detection of porcine reproductive and respiratory syndrome virus in the ovary of gilts culled due to reproductive disturbances. Comp Clin Pathol 24:903–910

    Article  CAS  Google Scholar 

  • Watanabe T, Inoue S, Ogawa S, Ishii Y, Hiroi H, Ikeda K, Orimo A, Muramatsu M (1997) Agonistic effect of tamoxifen is dependent on cell type, pre-promoter context, and estrogen receptor subtype: functional difference between estrogen receptors α and β. Biochem Biophys Res Commun 236:140–145

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Financial support for the study was provided by a Grant for International Research Integration: Chula Research Scholar, Ratchadaphiseksomphot Endowment Fund. D. Phoophitphong is a grantee of the Royal Golden Jubilee (RGJ) Ph.D. Program, the Thailand Research Fund.

Author information

Authors and Affiliations

  1. Department of Obstetrics, Gynaecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Padet Tummaruk, Nusjira Boonwong, Wannapha Chumthong, Wikanda Saengthong, Suthinee Jiraphan & Duangkamol Phoophitphong

  2. Department of Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Sayamon Srisuwatanasagul

Authors
  1. Padet Tummaruk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nusjira Boonwong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wannapha Chumthong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wikanda Saengthong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Suthinee Jiraphan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Duangkamol Phoophitphong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sayamon Srisuwatanasagul
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Padet Tummaruk.

Ethics declarations

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tummaruk, P., Boonwong, N., Chumthong, W. et al. Expression of oestrogen receptor α in the endometrium of porcine reproductive and respiratory syndrome virus-infected gilts. Comp Clin Pathol 25, 549–554 (2016). https://doi.org/10.1007/s00580-016-2226-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-016-2226-0

Keywords

Search

Navigation