Abstract
Retinoblastoma (RB) is a childhood eye tumor, caused by the RB1 gene mutation. Since RB is a rapidly proliferating tumor, the patient presents with a Group-D/E tumor at the time of diagnosis. Enucleation is preferred in most unilateral cases to prevent metastasis. Various cell lines have been established to study the tumor’s growth pattern and target the cancer cells. The commonly used cell lines are WERI-Rb-1 and Y79, both isolated from the primary tumor of RB. Cell lines established from the metastatic site of RB have not been characterized before. In this study, we have characterized NCC-RbC-51, derived from RB tumor to cervical lymph node site and investigated its potential to represent a highly aggressive and metastatic tumor. We compared the proliferative and invasive properties of NCC-RbC-51 with a cell line isolated from the primary site, WERI-Rb-1. NCC-RbC-51 had higher rates of proliferation and apoptosis and had better invasive ability. Copy number variation analysis and the pathways predicted from these show that the pathways altered in NCC-RbC-51 could contribute to its metastatic nature. In all, the results suggest that NCC-RbC-51, a cell line isolated from metastatic site, could be a potential model to study aggressive/invasive RB.
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Acknowledgements
We would like to acknowledge the Department of Biotechnology, India for funding the work (EXOSOMAL microRNA AS BIOMARKERS OF EARLY AND LATE STAGE RETINOBLASTOMA BT/PR8606/AGR/36/781/2013). We would like to acknowledge Dr. Masako Inomata, the originator of the NCC-RbC-51 cell line.
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418_2019_1832_MOESM3_ESM.tif
The untreated and Annexin PI stained RB cell lines, WERI-Rb-1 (B) and NCC-RbC-51 (A) showing the apoptotic profile of the two cell lines. (Q1 - Dead cell population, Annexin V–PI + , Q2 - Late apoptotic population, Annexin V + PI + , Q3 - Non-apoptotic population, Annexin V– PI–, Q4 - Early apoptotic population Annexin V + PI–) (TIFF 257 kb)
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Ravishankar, H., Mangani, A.S., Shankar, M.B. et al. Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site. Histochem Cell Biol 153, 101–109 (2020). https://doi.org/10.1007/s00418-019-01832-1
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DOI: https://doi.org/10.1007/s00418-019-01832-1