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Ectopic Expression of Fagopyrum tataricum FtMYB12 Improves Cold Tolerance in Arabidopsis thaliana

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Abstract

MYB transcription factors play important roles in the abiotic stress response in plants, but their characteristics and functions in buckwheat (Fagopyrum tataricum) have not been fully investigated. Here, a novel R2R3-type MYB gene, designated FtMYB12, was isolated from the cultivated tartary buckwheat F. tataricum. Using quantitative real-time PCR, we found that the FtMYB12 was greatly induced by low temperature. Sub-localization and yeast transactivity assay demonstrated that the FtMYB12 gene encodes a nuclear transcription activator. Overexpression of FtMYB12 in transgenic Arabidopsis plants resulted in enhanced cold tolerance. The FtMYB12 overexpressing Arabidopsis lines showed higher root length and had elevated levels of proline content and lower levels of malondialdehyde under cold stress conditions compared to the wild-type plants. The results revealed that FtMYB12 may play an essential role in regulation of cold stress-responsive signaling in F. tataricum.

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Acknowledgments

This research was supported by the Key Project of Science and Technology of Sichuan, China (Grant No. 04NG001-015, ‘‘Protection and exploitation of wild-type buckwheat germplasm resource’’), the grant from the National Transgenic Program (2013ZX08005-004) and National Natural Science Foundation of China (Grant No. 31372361).

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Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Meiliang Zhou, Chenglong Wang, Zhanmin Sun, Yixiong Tang & Yanmin Wu

  2. School of Life Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, China

    Meiliang Zhou, Chenglong Wang, Leipeng Qi, Xiongbang Yang & Jirong Shao

  3. Department of Food Science, Sichuan Tourism University, Chengdu, 610100, Sichuan, China

    Yu Tang

  4. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Room 211, Zhongguancun South Street No 12, Haidian District, Beijing, 100081, China

    Yanmin Wu

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  1. Meiliang Zhou
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Correspondence to Jirong Shao or Yanmin Wu.

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

344_2014_9472_MOESM1_ESM.jpg

Fagopyrum tataricum FtMYB12 sequence (red represents exon sequence, dark represents intron sequence). Supplementary material 1 (JPEG 157 kb)

FtMYB12 gene expression in 9 trangenic FtMYB12 overexpressing Arabidopsis lines. Supplementary material 2 (JPEG 31 kb)

344_2014_9472_MOESM3_ESM.jpg

COR15a gene expression in 9 trangenic FtMYB12 overexpressing Arabidopsis lines under room temperature (25 °C). Supplementary material 3 (JPEG 33 kb)

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Zhou, M., Wang, C., Qi, L. et al. Ectopic Expression of Fagopyrum tataricum FtMYB12 Improves Cold Tolerance in Arabidopsis thaliana . J Plant Growth Regul 34, 362–371 (2015). https://doi.org/10.1007/s00344-014-9472-7

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  • DOI: https://doi.org/10.1007/s00344-014-9472-7

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