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Industrial robustness linked to the gluconolactonase from Zymomonas mobilis

  • Applied microbial and cell physiology
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Abstract

The physiological characteristics and the potential gluconolactone production of the gluconolactonase-deficient strain, Zymomonas mobilis ZM4 gnlΔ, were investigated via growth inhibitory assay and biotransformation of glucose and fructose into gluconolactone and sorbitol, respectively. The results of ethanol fermentation studies performed in the presence of high concentration of glucose (>200 g l−1) under fermentative or aerobic conditions indicated that a significant reduction of volumetric ethanol productivity from the strain of ZM4 gnlΔ was noticeable due to the reduced rates of specific growth, sugar uptake, and biomass yield as compared with those of the parental strain ZM4. The biotransformation prepared at pH 6.0 using the permeabilized cell indicated that gluconic acid from ZM4 gnlΔ was still produced as a major product (67 g l−1) together with sorbitol (65 g l−1) rather than gluconolactone after 24 h. Only small amount of gluconolactone was transiently overproduced up to 9 g l−1, but at the end of biotransformation, all gluconolactone were oxidized into gluconic acid. This indicated that autolysis of gluconolactone at the pH led to such results despite under gluconolactonase inactivation conditions. The physiological characteristics of ZM4 gnlΔ was further investigated under various stress conditions, including suboptimal pH (3.5~6.0), temperature (25~40 °C), and presence of growth inhibitory molecules including hydrogen peroxide, ethanol, acetic acid, furfural, and so forth. The results indicated that ZM4 gnlΔ was more susceptible at high glucose concentration, low pH of 3.5, and high temperature of 40 °C and in the presence of 4 mM H2O2 comparing with ZM4. Therefore, the results were evident that gluconolactonase in Z. mobilis contributed to industrial robustness and anti-stress regulation.

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Authors and Affiliations

  1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Kensington, 2052, Australia

    Alfonsus Alvin, Brett A. Neilan & Young Jae Jeon

  2. Department of Microbiology, Pukyong National University, Busan, 608-737, Republic of Korea

    Junhyun Kim & Young Jae Jeon

  3. Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea

    Gwi-Taek Jeong

  4. Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, New Territories, Hong Kong

    Yiu Fai Tsang

  5. Department of Environment and Energy, Sejong University, Seoul, 143-747, Republic of Korea

    Eilhann E. Kwon

  6. School of Environmental and Life Science, The University of Newcastle, Newcastle, Australia

    Brett A. Neilan

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  1. Alfonsus Alvin
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  2. Junhyun Kim
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  3. Gwi-Taek Jeong
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  7. Young Jae Jeon
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Correspondence to Young Jae Jeon.

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Funding

This research as carried out with support of the National Research Foundation of Korea (NRF) under Grant No. NRF-2015R1D1A1A01056794.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Alvin, A., Kim, J., Jeong, GT. et al. Industrial robustness linked to the gluconolactonase from Zymomonas mobilis . Appl Microbiol Biotechnol 101, 5089–5099 (2017). https://doi.org/10.1007/s00253-017-8248-y

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  • DOI: https://doi.org/10.1007/s00253-017-8248-y

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