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Multiple paths of electron flow to current in microbial electrolysis cells fed with low and high concentrations of propionate

  • Environmental biotechnology
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Abstract

Microbial electrolysis cells (MECs) provide a viable approach for bioenergy generation from fermentable substrates such as propionate. However, the paths of electron flow during propionate oxidation in the anode of MECs are unknown. Here, the paths of electron flow involved in propionate oxidation in the anode of two-chambered MECs were examined at low (4.5 mM) and high (36 mM) propionate concentrations. Electron mass balances and microbial community analysis revealed that multiple paths of electron flow (via acetate/H2 or acetate/formate) to current could occur simultaneously during propionate oxidation regardless of the concentration tested. Current (57–96 %) was the largest electron sink and methane (0–2.3 %) production was relatively unimportant at both concentrations based on electron balances. At a low propionate concentration, reactors supplemented with 2-bromoethanesulfonate had slightly higher coulombic efficiencies than reactors lacking this methanogenesis inhibitor. However, an opposite trend was observed at high propionate concentration, where reactors supplemented with 2-bromoethanesulfonate had a lower coulombic efficiency and there was a greater percentage of electron loss (23.5 %) to undefined sinks compared to reactors without 2-bromoethanesulfonate (11.2 %). Propionate removal efficiencies were 98 % (low propionate concentration) and 78 % (high propionate concentration). Analysis of 16S rRNA gene pyrosequencing revealed the dominance of sequences most similar to Geobacter sulfurreducens PCA and G. sulfurreducens subsp. ethanolicus. Collectively, these results provide new insights on the paths of electron flow during propionate oxidation in the anode of MECs fed with low and high propionate concentrations.

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Acknowledgments

This work was sponsored by a Ph.D. fellowship, a Global Research Partnership-Collaborative Fellows Award (GRP-CF-2011-15-S), and Center Competitive Funding (FCC/1/1971-05-01) to P.E.S. from King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Bioscience Core Laboratory at KAUST for 454 pyrosequencing.

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  1. Biological and Environmental Sciences and Engineering Division, Water Desalination and Reuse Research Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

    Ananda Rao Hari, Krishna P. Katuri, Eduardo Gorron & Pascal E. Saikaly

  2. Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA, USA

    Bruce E. Logan

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  1. Ananda Rao Hari
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  2. Krishna P. Katuri
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  3. Eduardo Gorron
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Correspondence to Pascal E. Saikaly.

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Hari, A.R., Katuri, K.P., Gorron, E. et al. Multiple paths of electron flow to current in microbial electrolysis cells fed with low and high concentrations of propionate. Appl Microbiol Biotechnol 100, 5999–6011 (2016). https://doi.org/10.1007/s00253-016-7402-2

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