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A chemometric study on phenolic profiles and biological properties of cotton honeys from Turkey

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Abstract

Honey, a nutritious and healthy product, has recently been the focus of research due to its characteristic chemical composition and a wide variety of biological properties. The current study aims to investigate the phenolic profiles and tyrosinase, acetylcholinesterase (AChE), urease and butyrylcholinesterase (BChE) enzymes inhibitory, anti-inflammatory, antioxidant, antimicrobial and quorum sensing inhibition activities of twelve cotton honeys in 2 different regions from Turkey with the chemometric approach. The phenolic profile was determined by HPLC–DAD and gallic acid (8.95–84.47 μg/g) and p-hydroxybenzoic acid (0.21–1.45 μg/g) were detected in all cotton honeys studied. The SB-coded cotton honey from Şanlıurfa showed the highest activity in β-carotene linoleic acid (IC50: 5.94 ± 0.25 mg/mL), ABTS (IC50: 12.95 ± 0.49 mg/mL), CUPRAC (A0.50: 8.65 ± 0.41 mg/mL), metal chelating (IC50: 5.46 ± 0.28 mg/mL), AChE (13.65 ± 0.21%) and BChE (9.78 ± 0.11%) assays. The SE-coded cotton honey from Şanlıurfa was found to be the strongest in DPPH (IC50: 37.62 ± 1.40 mg/mL), anti-QS (44 mm zone) and swarming motility inhibition (30.47%) activities. Among all the other cotton honeys studied, AK1-coded cotton honey from Adana showed the highest antimicrobial activity against S. aureus (22 mm zone), L. monocytogenes (13 mm zone) bacteria and C. albicans (13 mm zone), C. tropicalis (15 mm zone) strains, and anti-inflammatory activity against COX-2 (16.59 ± 0.24%) and COX-1 (42.71 ± 0.56%). According to the PCA and HCA analysis results of cotton honeys based on phenolic compounds and biological activities, it is seen that cotton honeys from Adana and Şanlıurfa were clustered separately.

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Acknowledgements

The authors would like to thank the Turkish Association of Beekeepers (TAB) and Mr Ziya Şahin for providing the cotton honeys. This study was supported by grants from the Ministry of Agriculture and Forestry, Turkey and the General Directorate of Agricultural Research and Policies (TAGEM 17/AR-GE/13).

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Authors and Affiliations

  1. Department of Chemistry and Chemical Processing Technologies, Muğla Vocational School, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    Fatih Çayan & Gülsen Tel-Çayan

  2. Department of Chemistry, Faculty of Science, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    Meltem Taş-Küçükaydın & Mehmet Emin Duru

  3. Department of Medical Services and Techniques, Köyceğiz Vocational School of Health Services, Muğla Sıtkı Koçman University, Köyceğiz, 48000, Muğla, Turkey

    Selçuk Küçükaydın

  4. Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    Cansu Korkmaz

  5. Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, 48147, Mugla, Turkey

    Özgür Ceylan

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  1. Fatih Çayan
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Correspondence to Gülsen Tel-Çayan.

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Çayan, F., Tel-Çayan, G., Taş-Küçükaydın, M. et al. A chemometric study on phenolic profiles and biological properties of cotton honeys from Turkey. Eur Food Res Technol 249, 2241–2252 (2023). https://doi.org/10.1007/s00217-023-04286-x

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