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Diversity, distribution, and bioprospecting potentials of carotenogenic yeast from mangrove ecosystem

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Abstract

Microbial production of carotenoids has gained significant interest for its cost-effectiveness and sustainable nature. This study focuses on 47 red-pigmented yeasts isolated from sediments and plant parts of 13 species of mangrove trees. The relative abundance and distribution of these yeasts varied with plant species and plant parts. The highest number of red yeasts was associated with the mangrove plant Avicennia officinalis (32%). Notably, the leaves harbored the highest percentage (45%) of carotenogenic yeasts, and definite compartmentalization of these yeast species was noticed in mangrove plant parts. All the isolates were molecularly identified and they belonged to the genera of Rhodotorula, Rhodosporidiobolus, and Cryptococcus. The diversity of the pigmented yeasts isolated from A. officinalis was found to be the greatest. Among these strains, Rhodotorula mucilaginosa PV 8 was identified as the most potent producer of carotenoid pigment. Under optimized conditions of physical parameters − 28 °C, pH 5, and 15% salinity led to biomass production of 9.2 ± 0.12 g/L DCW and a pigment yield of 194.78 µg/g. The pigment produced by PV 8 was identified as β-carotene by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). This β-carotene demonstrated strong antioxidant activity. Moreover, the carotenoid displayed promising antibacterial activity against multidrug-resistant organisms, including Aeromonas sp. and Vibrio sp. In vitro studies revealed the probiotic traits of PV 8. The cytotoxicity of R. mucilaginosa PV 8 was assessed in the invertebrate model Artemia salina and the survival rate showed that it was non-toxic. Furthermore, the β-carotene from PV 8 demonstrated the ability to transfer its vibrant color to various food products, maintaining color stability even under varied conditions. This research underscores the potential of R. mucilaginosa PV 8, as a versatile and valuable resource for the production of carotenoids.

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Acknowledgements

This work was supported by the Kerala University of Fisheries and Ocean Studies aided research project (KARP) scheme, Order No: DoR (1)/5587/2021. The authors would also like to acknowledge the support rendered by Dr. S. Suresh Kumar, The Dean, of the Faculty of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies.

Funding

This work was funded by the grant from the Kerala University of Fisheries and Ocean Studies. KUFOS KARP,DoR (1)/5587/2021

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  1. Department of Marine Biosciences, Faculty of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies, Panangad, Kochi, Kerala, 682506, India

    K. A. Nimsi, K. Manjusha & M. N. Farzana

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Contributions

Study conception and design: K A Nimsi, K Manjusha. Material preparation and analysis: K A Nimsi, M.N. Farzana. Data collection: K A Nimsi. The draft of the manuscript was written by K A Nimsi. K Manjusha edited and approved the manuscript.

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Correspondence to K. Manjusha.

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Communicated by Nischitha R.

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Nimsi, K.A., Manjusha, K. & Farzana, M.N. Diversity, distribution, and bioprospecting potentials of carotenogenic yeast from mangrove ecosystem. Arch Microbiol 206, 189 (2024). https://doi.org/10.1007/s00203-024-03879-8

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