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Cultivable endophytic bacteria from heavy metal(loid)-tolerant plants

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Abstract

To evaluate the interactions among endophytes, plants and heavy metal/arsenic contamination, root endophytic bacteria of Prosopis laevigata (Humb and Bonpl. ex Willd) and Sphaeralcea angustifolia grown in a heavy metal(loid)-contaminated zone in San Luis Potosi, Mexico, were isolated and characterized. Greater abundance and species richness were found in Prosopis than in Sphaeralcea and in the nutrient Pb–Zn-rich hill than in the poor nutrient and As–Cu-rich mine tailing. The 25 species identified among the 60 isolates formed three groups in the correspondence analysis, relating to Prosopis/hill (11 species), Prosopis/mine tailing (4 species) and Sphaeralcea/hill (4 species), with six species ungrouped. Most of the isolates showed high or extremely high resistance to arsenic, such as ≥100 mM for As(V) and ≥20 mM for As(III), in mineral medium. These results demonstrated that the abundance and community composition of root endophytic bacteria were strongly affected by the concentration and type of the heavy metals and metalloids (arsenic), as well as the plant species.

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Acknowledgments

We thank A. Patiño-Siliciano for identifying the plant samples. This study was funded by the Projects SIP 20130722, 20130828 and 20140124 authorized by IPN. B.R.P. received scholarships support from the CONACyT and PIFI. M.S.V.-M., F.N.R.-O. and E.T.W. appreciate the scholarships of COFAA and EDI-IPN and SNI-CONACyT.

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  1. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Ciudad de México, Mexico

    Brenda Román-Ponce, Juan Ramos-Garza, María Soledad Vásquez-Murrieta, Flor Nohemí Rivera-Orduña, Paulina Estrada-de los Santos & En Tao Wang

  2. State Key Laboratory of Agrobiotechnology, College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing, 100193, China

    Wen Feng Chen

  3. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China

    Jun Yan

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  1. Brenda Román-Ponce
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Román-Ponce, B., Ramos-Garza, J., Vásquez-Murrieta, M.S. et al. Cultivable endophytic bacteria from heavy metal(loid)-tolerant plants. Arch Microbiol 198, 941–956 (2016). https://doi.org/10.1007/s00203-016-1252-2

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