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Production and Characterization of Siderophores and its Application in Arsenic Removal from Contaminated Soil

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Abstract

Siderophores are small molecular weight extracellular organic compounds secreted by microorganisms under iron-starved conditions, used by them to chelate and solubilize iron. Though they are specific ferric iron chelator, but is reported that they bind other metals also, such as divalent heavy metals and actinides because of potentially high metal-siderophore stability constants. Thus metal contaminant fate and transport in subsurface environment can be heavily influenced by siderophores. This approach can be successfully used in removing many toxic metals off the soil which poses a serious health threat. Our research focuses on the correlation between cell growth and siderophore production and chemical characterization of the siderophore type. Its also documents the development of an assay method for the screening of different metals for complexation with siderophores based on the Chrome Azurol S (CAS) assay. The present research aims at batch scale mobilization of arsenic from arsenic contaminated soils using siderophore produced by P. azotoformans and thus evaluating its efficiency as compared to Ethylene Diamine Tetra Acetic Acid (EDTA), Citric Acid (CA) for the same. FT-IR spectroscopic studies were carried out to determine the interaction between soil, arsenic and siderophore. Results have shown that the cell growth and siderophore production are inversely related. Characterization of siderophore produced by P. azotoformans has revealed that it is of mixed-type catecholate and hydroxamate. Siderophore was found to complex with heavy metals like Cadmium, Lead, Nickel, Arsenic (III, V), Aluminium, Magnesium Zinc, Copper, Cobalt, Strontium other than Iron. Five washings by siderophore, EDTA, CA removed almost 92.8%, 77.3%, 70.0% arsenic respectively as compared to only 33.8% removal by control. Washing of arsenic contaminated soil with tap water revealed that ≈ 65.8% of arsenic in contaminated soil is in freely available or weakly bound form. The IR spectra revealed that hydrogen bonding exists between siderophore, arsenic and soil. Encouraging results of arsenic removal by biomolecule-siderophore can lead to an emerging tool brimming with opportunities for environmental clean up.

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Acknowledgments

The authors acknowledge Dr. Sukumar Devotta, Director, NEERI, Nagpur for encouragement and valuable suggestions. We specially thank Dr. Srimant Kagne and Mr Ravi Chatti for interpretation of FT-IR results. Thanks go to Ms Prachi Joshi and Ms Radha for their suggestions and constant support. The authors are thankful to the reviewers for their constructive comments.

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  1. Environmental Biotechnology Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, India

    Anupa Nair, Asha A. Juwarkar & Sanjeev K. Singh

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  1. Anupa Nair
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  2. Asha A. Juwarkar
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  3. Sanjeev K. Singh
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Correspondence to Asha A. Juwarkar.

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Nair, A., Juwarkar, A.A. & Singh, S.K. Production and Characterization of Siderophores and its Application in Arsenic Removal from Contaminated Soil. Water Air Soil Pollut 180, 199–212 (2007). https://doi.org/10.1007/s11270-006-9263-2

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