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Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast

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Abstract

Faithful chromosome segregation during mitosis requires the correct assembly of kinetochore on the centromere. CENP-A is a variant of histone H3, which specializes the centromere region on chromatin and mediates the kinetochore assembly. The Mis18 complex plays a critical role in initiating the centromere loading of the newly-synthesized CENP-A. However, it remains unclear how Mis18 complex (spMis18, spMis16 and spMis19) is located to the centromere to license the recruitment of Cnp1CENP-A in Schizosaccharomyces pombe. We found that spMis18 directly binds to nucleosomal DNA through its extreme C-terminus and interacts with H2A–H2B dimer via the acidic region on the surface of its Yippee-like domain. Live-cell imaging confirmed that mutation of the acidic region and deletion of the extreme C-terminus significantly impairs the localization of spMis18 and Cnp1 to the centromere and delays chromosome segregation during mitosis. Our findings illustrate that the interaction of spMis18 with histone H2A–H2B and DNA plays important roles in the recruitment of spMis18 and Cnp1 to the centromere in fission yeast.

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Abbreviations

CD:

Circular dichroism

CCD:

Charge-coupled device

CENP:

Centromere protein

FL:

Full length

GADPH:

Glyceraldehyde-3-phosphate dehydrogenase

GFP:

Green fluorescent protein

GST:

Glutathione S-transferase

HJURP:

Holliday junction recognition protein

IPTG:

Isopropyl 1-β-d-galactopyranoside

LB:

Luria-Bertani

M18BP1:

Mis18 binding protein 1

NCP:

Nucleosome

Ni–NTA:

Nickel–nitriloacetic acid

PAGE:

Polyacrylamide gel electrophoresis

PCR:

Polymerase chain reaction

PMSF:

Phenylmethylsulfonyl fluoride

RSF1:

Remodeling and spacing factor 1

SAD:

Single-wavelength anomalous dispersion

SEC:

Size-exclusion chromatography

MALS:

Multiangle light scattering

SDS:

Sodium dodecyl sulfate

SSRF:

Shanghai synchrotron radiation facility

TEV:

Tobacco etch virus

Tris:

Tris(hydroxymethyl)aminomethane

Trx:

Thioredoxin

WT:

Wild type

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Acknowledgements

We thank the staff at beamline BL17U1 of the Shanghai Synchrotron Radiation Facility of the National Facility for Protein Science in Shanghai for assistance with data collection. This work was supported by the National Key Research and Development Program of China (2017YFA0503600, 2016YFA0400903), the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (31621002), the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology (2017FXCX004), Users with Excellence Project of Hefei Science Center CAS (2018HSC-UE001), the National Natural Science Foundation of China (U1532109, 91853133 to JZ, 31700671 to XZ), and USTC Research Funds of the Double First-Class Initiative.

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Author notes

  1. Min Zhang and Fan Zheng contribute equally to this work.

Authors and Affiliations

  1. Hefei National Laboratory for Physical Sciences at Microscale, the First Affiliated Hospital of USTC, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, 230026, Anhui, China

    Min Zhang, Fan Zheng, Yujie Xiong, Chen Shao, Chengliang Wang, Minhao Wu, Xiaojia Niu, Fenfen Dong, Xuan Zhang, Chuanhai Fu & Jianye Zang

  2. Key Laboratory of Structural Biology, Chinese Academy of Sciences, Hefei, 230026, Anhui, China

    Min Zhang, Fan Zheng, Yujie Xiong, Chen Shao, Chengliang Wang, Minhao Wu, Xiaojia Niu, Fenfen Dong, Xuan Zhang, Chuanhai Fu & Jianye Zang

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  1. Min Zhang
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Contributions

JZ, XZ provided the scientific direction and the overall experimental design for the studies. CF and FZ provided the experimental design for in vivo studies. MZ, XZ and CS performed the biochemical experiments. MZ carried out the in vivo biochemistry experiments. FZ and MZ constructed the yeast strains. FZ performed live-cell imaging. MZ analyzed the imaging results with the help from FZ. XN and FD provided assistance in the biochemical experiments in fission yeast. CS, CW and MW were responsible for the crystal structure studies. MZ, FZ, XZ, CF and JZ wrote the manuscript.

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Correspondence to Xuan Zhang, Chuanhai Fu or Jianye Zang.

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Zhang, M., Zheng, F., Xiong, Y. et al. Centromere targeting of Mis18 requires the interaction with DNA and H2A–H2B in fission yeast. Cell. Mol. Life Sci. 78, 373–384 (2021). https://doi.org/10.1007/s00018-020-03502-1

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